Metallo-β-lactamase production by Pseudomonas otitidis

A species-related trait

Maria Cristina Thaller, Luisa Borgianni, Gustavo Di Lallo, Yunsop Chong, Kyungwon Lee, Joseph Dajcs, David Stroman, Gian Maria Rossolini

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Susceptibility to several β-lactams and β-lactamase production was investigated in a collection of 20 strains of Pseudomonas otitidis, a new Pseudomonas species that has been recently recognized in association with otic infections in humans. All strains appeared to be susceptible to piperacillin, cefotaxime, ceftazidime, and aztreonam, while resistance or decreased susceptibility to carbapenems was occasionally observed. All strains were found to express metallo-β-lactamase (MBL) activity and to carry a new subclass B3 MBL gene, named blaPOM, that appeared to be highly conserved in this species. P. otitidis, therefore, is the first example of a pathogenic Pseudomonas species endowed with a resident MBL. The POM-1 protein from P. otitidis type strain MCC10330 exhibits the closest similarity (60 to 64%) to the L1 MBL of Stenotrophomonas maltophilia. Expression in Escherichia coli and Pseudomonas aeruginosa revealed that, similar to L1 and other subclass B3 MBLs, POM-1 confers decreased susceptibility or resistance to carbapenems, penicillins, and cephalosporins but not to aztreonam. Expression of the POM MBL in P. otitidis is apparently constitutive and, in most strains, does not confer a carbapenem-resistant phenotype. However, a strong inoculum size effect was observed for carbapenem MICs, and carbapenem-resistant mutants could be readily selected upon exposure to imipenem, suggesting that carbapenem-based regimens should be considered with caution for P. otitidis infections.

Original languageEnglish
Pages (from-to)118-123
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume55
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1

Fingerprint

Carbapenems
Pseudomonas
Aztreonam
Stenotrophomonas maltophilia
Lactams
Piperacillin
Ceftazidime
Cefotaxime
Imipenem
Cephalosporins
Infection
Penicillins
Pseudomonas aeruginosa
Ear
Escherichia coli
Phenotype
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Thaller, M. C., Borgianni, L., Di Lallo, G., Chong, Y., Lee, K., Dajcs, J., ... Rossolini, G. M. (2011). Metallo-β-lactamase production by Pseudomonas otitidis: A species-related trait. Antimicrobial Agents and Chemotherapy, 55(1), 118-123. https://doi.org/10.1128/AAC.01062-10
Thaller, Maria Cristina ; Borgianni, Luisa ; Di Lallo, Gustavo ; Chong, Yunsop ; Lee, Kyungwon ; Dajcs, Joseph ; Stroman, David ; Rossolini, Gian Maria. / Metallo-β-lactamase production by Pseudomonas otitidis : A species-related trait. In: Antimicrobial Agents and Chemotherapy. 2011 ; Vol. 55, No. 1. pp. 118-123.
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Thaller, MC, Borgianni, L, Di Lallo, G, Chong, Y, Lee, K, Dajcs, J, Stroman, D & Rossolini, GM 2011, 'Metallo-β-lactamase production by Pseudomonas otitidis: A species-related trait', Antimicrobial Agents and Chemotherapy, vol. 55, no. 1, pp. 118-123. https://doi.org/10.1128/AAC.01062-10

Metallo-β-lactamase production by Pseudomonas otitidis : A species-related trait. / Thaller, Maria Cristina; Borgianni, Luisa; Di Lallo, Gustavo; Chong, Yunsop; Lee, Kyungwon; Dajcs, Joseph; Stroman, David; Rossolini, Gian Maria.

In: Antimicrobial Agents and Chemotherapy, Vol. 55, No. 1, 01.01.2011, p. 118-123.

Research output: Contribution to journalArticle

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T2 - A species-related trait

AU - Thaller, Maria Cristina

AU - Borgianni, Luisa

AU - Di Lallo, Gustavo

AU - Chong, Yunsop

AU - Lee, Kyungwon

AU - Dajcs, Joseph

AU - Stroman, David

AU - Rossolini, Gian Maria

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