Methane concentrations and methanotrophic community structure influence the response of soil methane oxidation to nitrogen content in a temperate forest

Inyoung Jang, Seunghoon Lee, Kyounung Duk Zoh, Hojeong Kang

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Methane oxidation in forest soils removes atmospheric CH4. Many studies have determined methane uptake rates and their controlling variables, yet the microorganisms involved have rarely been assessed simultaneously over the longer term. We measured methane uptake rates and the community structure of methanotrophic bacteria in temperate forest soil (sandy clay loam) on a monthly basis for two years in South Korea. Methane uptake rates at the field site did not show any seasonal patterns, and net uptake occurred throughout both years. In situ uptake rates and uptake potentials determined in the laboratory were 2.92 ± 4.07 mg CH4 m-2 day-1 and 51.6 ± 45.8 ng CH4 g-1 soil day-1, respectively. Contrary to results from other studies, in situ oxidation rates were positively correlated with soil nitrate concentrations. Short-term experimental nitrate addition (0.20-1.95 μg N g-1 soil) significantly stimulated oxidation rates under low methane concentrations (1.7-2.0 ppmv CH4), but significantly inhibited oxidation under high methane concentrations (300 ppmv CH4). We analyzed the community structures of methanotrophic bacteria using a DNA-based fingerprinting method (T-RFLP). Type II methanotrophs dominated under low methane concentrations while Type I methanotrophs dominated under high methane concentrations. Nitrogen addition selectively inhibited Type I methanotrophic bacteria. Overall, the results of this study indicate that the effects of inorganic N on methane uptake depend on methane concentrations and that such a response is related to the dissimilar activation or inhibition of different types of methanotrophic bacteria.

Original languageEnglish
Pages (from-to)620-627
Number of pages8
JournalSoil Biology and Biochemistry
Volume43
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

Fingerprint

Methane
temperate forests
temperate forest
nitrogen content
methane
community structure
Nitrogen
Soil
oxidation
nitrogen
soil
Bacteria
bacterium
Nitrates
forest soil
methanotrophs
bacteria
Forests
nitrate
sandy clay loam

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Soil Science

Cite this

@article{6668554d25914da3ac38395d7b949b72,
title = "Methane concentrations and methanotrophic community structure influence the response of soil methane oxidation to nitrogen content in a temperate forest",
abstract = "Methane oxidation in forest soils removes atmospheric CH4. Many studies have determined methane uptake rates and their controlling variables, yet the microorganisms involved have rarely been assessed simultaneously over the longer term. We measured methane uptake rates and the community structure of methanotrophic bacteria in temperate forest soil (sandy clay loam) on a monthly basis for two years in South Korea. Methane uptake rates at the field site did not show any seasonal patterns, and net uptake occurred throughout both years. In situ uptake rates and uptake potentials determined in the laboratory were 2.92 ± 4.07 mg CH4 m-2 day-1 and 51.6 ± 45.8 ng CH4 g-1 soil day-1, respectively. Contrary to results from other studies, in situ oxidation rates were positively correlated with soil nitrate concentrations. Short-term experimental nitrate addition (0.20-1.95 μg N g-1 soil) significantly stimulated oxidation rates under low methane concentrations (1.7-2.0 ppmv CH4), but significantly inhibited oxidation under high methane concentrations (300 ppmv CH4). We analyzed the community structures of methanotrophic bacteria using a DNA-based fingerprinting method (T-RFLP). Type II methanotrophs dominated under low methane concentrations while Type I methanotrophs dominated under high methane concentrations. Nitrogen addition selectively inhibited Type I methanotrophic bacteria. Overall, the results of this study indicate that the effects of inorganic N on methane uptake depend on methane concentrations and that such a response is related to the dissimilar activation or inhibition of different types of methanotrophic bacteria.",
author = "Inyoung Jang and Seunghoon Lee and Zoh, {Kyounung Duk} and Hojeong Kang",
year = "2011",
month = "3",
day = "1",
doi = "10.1016/j.soilbio.2010.11.032",
language = "English",
volume = "43",
pages = "620--627",
journal = "Soil Biology and Biochemistry",
issn = "0038-0717",
publisher = "Elsevier Limited",
number = "3",

}

Methane concentrations and methanotrophic community structure influence the response of soil methane oxidation to nitrogen content in a temperate forest. / Jang, Inyoung; Lee, Seunghoon; Zoh, Kyounung Duk; Kang, Hojeong.

In: Soil Biology and Biochemistry, Vol. 43, No. 3, 01.03.2011, p. 620-627.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Methane concentrations and methanotrophic community structure influence the response of soil methane oxidation to nitrogen content in a temperate forest

AU - Jang, Inyoung

AU - Lee, Seunghoon

AU - Zoh, Kyounung Duk

AU - Kang, Hojeong

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Methane oxidation in forest soils removes atmospheric CH4. Many studies have determined methane uptake rates and their controlling variables, yet the microorganisms involved have rarely been assessed simultaneously over the longer term. We measured methane uptake rates and the community structure of methanotrophic bacteria in temperate forest soil (sandy clay loam) on a monthly basis for two years in South Korea. Methane uptake rates at the field site did not show any seasonal patterns, and net uptake occurred throughout both years. In situ uptake rates and uptake potentials determined in the laboratory were 2.92 ± 4.07 mg CH4 m-2 day-1 and 51.6 ± 45.8 ng CH4 g-1 soil day-1, respectively. Contrary to results from other studies, in situ oxidation rates were positively correlated with soil nitrate concentrations. Short-term experimental nitrate addition (0.20-1.95 μg N g-1 soil) significantly stimulated oxidation rates under low methane concentrations (1.7-2.0 ppmv CH4), but significantly inhibited oxidation under high methane concentrations (300 ppmv CH4). We analyzed the community structures of methanotrophic bacteria using a DNA-based fingerprinting method (T-RFLP). Type II methanotrophs dominated under low methane concentrations while Type I methanotrophs dominated under high methane concentrations. Nitrogen addition selectively inhibited Type I methanotrophic bacteria. Overall, the results of this study indicate that the effects of inorganic N on methane uptake depend on methane concentrations and that such a response is related to the dissimilar activation or inhibition of different types of methanotrophic bacteria.

AB - Methane oxidation in forest soils removes atmospheric CH4. Many studies have determined methane uptake rates and their controlling variables, yet the microorganisms involved have rarely been assessed simultaneously over the longer term. We measured methane uptake rates and the community structure of methanotrophic bacteria in temperate forest soil (sandy clay loam) on a monthly basis for two years in South Korea. Methane uptake rates at the field site did not show any seasonal patterns, and net uptake occurred throughout both years. In situ uptake rates and uptake potentials determined in the laboratory were 2.92 ± 4.07 mg CH4 m-2 day-1 and 51.6 ± 45.8 ng CH4 g-1 soil day-1, respectively. Contrary to results from other studies, in situ oxidation rates were positively correlated with soil nitrate concentrations. Short-term experimental nitrate addition (0.20-1.95 μg N g-1 soil) significantly stimulated oxidation rates under low methane concentrations (1.7-2.0 ppmv CH4), but significantly inhibited oxidation under high methane concentrations (300 ppmv CH4). We analyzed the community structures of methanotrophic bacteria using a DNA-based fingerprinting method (T-RFLP). Type II methanotrophs dominated under low methane concentrations while Type I methanotrophs dominated under high methane concentrations. Nitrogen addition selectively inhibited Type I methanotrophic bacteria. Overall, the results of this study indicate that the effects of inorganic N on methane uptake depend on methane concentrations and that such a response is related to the dissimilar activation or inhibition of different types of methanotrophic bacteria.

UR - http://www.scopus.com/inward/record.url?scp=78751569638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78751569638&partnerID=8YFLogxK

U2 - 10.1016/j.soilbio.2010.11.032

DO - 10.1016/j.soilbio.2010.11.032

M3 - Article

AN - SCOPUS:78751569638

VL - 43

SP - 620

EP - 627

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

IS - 3

ER -