Methanol extract of Cordyceps pruinosa inhibits in vitro and in vivo inflammatory mediators by suppressing NF-κB activation

Ki Mo Kim, Young-Guen Kwon, Hun Taeg Chung, Young Gab Yun, Hyun Ock Pae, Jeong A. Han, Kwon Soo Ha, Tae Woong Kim, Young Myeong Kim

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Cordyceps pruinosa has been used in traditional folk medicine to treat numerous diseases. The molecular mechanism of C. pruinosa pharmacological and biochemical actions of macrophages in inflammation has not been clearly elucidated. We examined how the methanol extract of C. pruinosa regulates production of IL-1β, TNF-α, nitric oxide (NO), and prostaglandin E 2 (PGE 2 ) in vitro and in vivo. The extract inhibits these inflammatory mediators in LPS-stimulated murine macrophage cell line RAW264.7 and primary macrophages, by suppressing gene expression of IL-1β, TNF-α, inducible nitric oxide synthase, and cyclooxygenase-2. Moreover, the extract suppresses the nuclear transcription factor NF-κB activation in LPS-stimulated RAW264.7 cells. Administration of the extract significantly decreases the plasma levels of these inflammatory mediators in LPS-injected mice. These results suggest that the C. pruinosa methanol extract suppresses inflammation through suppression of NF-κB-dependent inflammatory gene expression, suggesting that the C. pruinosa extract may be beneficial for treatment of endotoxin shock or sepsis.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalToxicology and Applied Pharmacology
Volume190
Issue number1
DOIs
Publication statusPublished - 2003 Jul 1

Fingerprint

Cordyceps
Macrophages
Methanol
Chemical activation
Traditional Medicine
Interleukin-1
Gene expression
Inflammation
Gene Expression
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Prostaglandins E
Endotoxins
Medicine
Shock
Sepsis
Nitric Oxide
Transcription Factors
Cells
Pharmacology

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology

Cite this

Kim, Ki Mo ; Kwon, Young-Guen ; Chung, Hun Taeg ; Yun, Young Gab ; Pae, Hyun Ock ; Han, Jeong A. ; Ha, Kwon Soo ; Kim, Tae Woong ; Kim, Young Myeong. / Methanol extract of Cordyceps pruinosa inhibits in vitro and in vivo inflammatory mediators by suppressing NF-κB activation. In: Toxicology and Applied Pharmacology. 2003 ; Vol. 190, No. 1. pp. 1-8.
@article{ba0ee4e0f56a4467b960776f2acd7e15,
title = "Methanol extract of Cordyceps pruinosa inhibits in vitro and in vivo inflammatory mediators by suppressing NF-κB activation",
abstract = "Cordyceps pruinosa has been used in traditional folk medicine to treat numerous diseases. The molecular mechanism of C. pruinosa pharmacological and biochemical actions of macrophages in inflammation has not been clearly elucidated. We examined how the methanol extract of C. pruinosa regulates production of IL-1β, TNF-α, nitric oxide (NO), and prostaglandin E 2 (PGE 2 ) in vitro and in vivo. The extract inhibits these inflammatory mediators in LPS-stimulated murine macrophage cell line RAW264.7 and primary macrophages, by suppressing gene expression of IL-1β, TNF-α, inducible nitric oxide synthase, and cyclooxygenase-2. Moreover, the extract suppresses the nuclear transcription factor NF-κB activation in LPS-stimulated RAW264.7 cells. Administration of the extract significantly decreases the plasma levels of these inflammatory mediators in LPS-injected mice. These results suggest that the C. pruinosa methanol extract suppresses inflammation through suppression of NF-κB-dependent inflammatory gene expression, suggesting that the C. pruinosa extract may be beneficial for treatment of endotoxin shock or sepsis.",
author = "Kim, {Ki Mo} and Young-Guen Kwon and Chung, {Hun Taeg} and Yun, {Young Gab} and Pae, {Hyun Ock} and Han, {Jeong A.} and Ha, {Kwon Soo} and Kim, {Tae Woong} and Kim, {Young Myeong}",
year = "2003",
month = "7",
day = "1",
doi = "10.1016/S0041-008X(03)00152-2",
language = "English",
volume = "190",
pages = "1--8",
journal = "Toxicology and Applied Pharmacology",
issn = "0041-008X",
publisher = "Academic Press Inc.",
number = "1",

}

Methanol extract of Cordyceps pruinosa inhibits in vitro and in vivo inflammatory mediators by suppressing NF-κB activation. / Kim, Ki Mo; Kwon, Young-Guen; Chung, Hun Taeg; Yun, Young Gab; Pae, Hyun Ock; Han, Jeong A.; Ha, Kwon Soo; Kim, Tae Woong; Kim, Young Myeong.

In: Toxicology and Applied Pharmacology, Vol. 190, No. 1, 01.07.2003, p. 1-8.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Methanol extract of Cordyceps pruinosa inhibits in vitro and in vivo inflammatory mediators by suppressing NF-κB activation

AU - Kim, Ki Mo

AU - Kwon, Young-Guen

AU - Chung, Hun Taeg

AU - Yun, Young Gab

AU - Pae, Hyun Ock

AU - Han, Jeong A.

AU - Ha, Kwon Soo

AU - Kim, Tae Woong

AU - Kim, Young Myeong

PY - 2003/7/1

Y1 - 2003/7/1

N2 - Cordyceps pruinosa has been used in traditional folk medicine to treat numerous diseases. The molecular mechanism of C. pruinosa pharmacological and biochemical actions of macrophages in inflammation has not been clearly elucidated. We examined how the methanol extract of C. pruinosa regulates production of IL-1β, TNF-α, nitric oxide (NO), and prostaglandin E 2 (PGE 2 ) in vitro and in vivo. The extract inhibits these inflammatory mediators in LPS-stimulated murine macrophage cell line RAW264.7 and primary macrophages, by suppressing gene expression of IL-1β, TNF-α, inducible nitric oxide synthase, and cyclooxygenase-2. Moreover, the extract suppresses the nuclear transcription factor NF-κB activation in LPS-stimulated RAW264.7 cells. Administration of the extract significantly decreases the plasma levels of these inflammatory mediators in LPS-injected mice. These results suggest that the C. pruinosa methanol extract suppresses inflammation through suppression of NF-κB-dependent inflammatory gene expression, suggesting that the C. pruinosa extract may be beneficial for treatment of endotoxin shock or sepsis.

AB - Cordyceps pruinosa has been used in traditional folk medicine to treat numerous diseases. The molecular mechanism of C. pruinosa pharmacological and biochemical actions of macrophages in inflammation has not been clearly elucidated. We examined how the methanol extract of C. pruinosa regulates production of IL-1β, TNF-α, nitric oxide (NO), and prostaglandin E 2 (PGE 2 ) in vitro and in vivo. The extract inhibits these inflammatory mediators in LPS-stimulated murine macrophage cell line RAW264.7 and primary macrophages, by suppressing gene expression of IL-1β, TNF-α, inducible nitric oxide synthase, and cyclooxygenase-2. Moreover, the extract suppresses the nuclear transcription factor NF-κB activation in LPS-stimulated RAW264.7 cells. Administration of the extract significantly decreases the plasma levels of these inflammatory mediators in LPS-injected mice. These results suggest that the C. pruinosa methanol extract suppresses inflammation through suppression of NF-κB-dependent inflammatory gene expression, suggesting that the C. pruinosa extract may be beneficial for treatment of endotoxin shock or sepsis.

UR - http://www.scopus.com/inward/record.url?scp=0038385029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038385029&partnerID=8YFLogxK

U2 - 10.1016/S0041-008X(03)00152-2

DO - 10.1016/S0041-008X(03)00152-2

M3 - Article

C2 - 12831777

AN - SCOPUS:0038385029

VL - 190

SP - 1

EP - 8

JO - Toxicology and Applied Pharmacology

JF - Toxicology and Applied Pharmacology

SN - 0041-008X

IS - 1

ER -