Methicillin-resistant staphylococcus aureus blood isolates harboring a novel pseudo-staphylococcal cassette chromosome mec element

Eun Jeong Yoon, Hyukmin Lee, Dokyun Kim, Jong Hee Shin, Jeong Hwan Shin, Seok Hoon Jeong

Research output: Contribution to journalArticle

Abstract

The aim of this work was to assess a novel pseudo-staphylococcal cassette chromosome mec (ΨSCCmec) element in methicillin-resistant Staphylococcus aureus (MRSA) blood isolates. Community-associated MRSA E16SA093 and healthcare-associated MRSA F17SA003 isolates were recovered from the blood specimens of patients with S. aureus bacteremia in 2016 and in 2017, respectively. Antimicrobial susceptibility was determined via the disk diffusion method, and SCCmec typing was conducted by multiplex polymerase chain reaction. Whole genome sequencing was carried out by single molecule real-time long-read sequencing. Both isolates belonged to sequence type 72 and agr-type I, and they were negative for Panton-Valentine leukocidin and toxic shock syndrome toxin. The spa-types of E16SA093 and F17SA003 were t324 and t2460, respectively. They had a SCCmec IV-like element devoid of the cassette chromosome recombinase (ccr) gene complex, designated as ΨSCCmecE16SA093. The element was manufactured from SCCmec type IV and the deletion of the ccr gene complex and a 7.0- and 31.9-kb portion of each chromosome. The deficiency of the ccr gene complex in the SCCmec unit is likely resulting in mobility loss, which would be an adaptive evolutionary mechanism. The dissemination of this clone should be monitored closely.

Original languageEnglish
Article number540
JournalFrontiers in Microbiology
Volume10
Issue numberMAR
DOIs
Publication statusPublished - 2019 Jan 1

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Methicillin-Resistant Staphylococcus aureus
Chromosomes
Recombinases
Genes
Chromosome Deletion
Multiplex Polymerase Chain Reaction
Septic Shock
Bacteremia
Staphylococcus aureus
Clone Cells
Genome
Delivery of Health Care

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Yoon, Eun Jeong ; Lee, Hyukmin ; Kim, Dokyun ; Shin, Jong Hee ; Shin, Jeong Hwan ; Jeong, Seok Hoon. / Methicillin-resistant staphylococcus aureus blood isolates harboring a novel pseudo-staphylococcal cassette chromosome mec element. In: Frontiers in Microbiology. 2019 ; Vol. 10, No. MAR.
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Methicillin-resistant staphylococcus aureus blood isolates harboring a novel pseudo-staphylococcal cassette chromosome mec element. / Yoon, Eun Jeong; Lee, Hyukmin; Kim, Dokyun; Shin, Jong Hee; Shin, Jeong Hwan; Jeong, Seok Hoon.

In: Frontiers in Microbiology, Vol. 10, No. MAR, 540, 01.01.2019.

Research output: Contribution to journalArticle

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