Methods for Evaluating the Caenorhabditis elegans Dauer State

Standard Dauer-Formation Assay Using Synthetic Daumones and Proteomic Analysis of O-GlcNAc Modifications

Jeeyong Lee, Kwang Youl Kim, Hyoe Jin Joo, Heekyeong Kim, Pan Young Jeong, Young-Ki Paik

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The dauer state is a non-feeding, alternative L3 state characterized by a number of distinctive metabolic and morphological changes. There are many naturally occurring dauer-inducing pheromones, termed daumones, that have been suggested by some to exhibit differences in dauer-inducing activity. Here, we have established a standard dauer-formation assay that uses synthetic daumones 1, 2, and 3, the three major daumones. To analyze the proteome of Caenorhabditis elegans in the dauer state, we focused on O-GlcNAc modification, a cytosolic modification of proteins that is known to interact either competitively or synergistically with protein phosphorylation. Protein O-GlcNAc modification is an important biological process in cells that can ensure the timely response to extracellular stimuli, such as daumone, and maintain cellular homeostasis. Establishing a standard method for assaying dauer formation using different synthetic daumones, and using differences in O-GlcNAcylated proteins during the dauer state to analyze the dauer proteome will lead to a better understanding of dauer biology of C. elegans in the context of animal longevity and adaptation under harsh environments.

Original languageEnglish
Pages (from-to)445-460
Number of pages16
JournalMethods in Cell Biology
Volume106
DOIs
Publication statusPublished - 2011 Dec 2

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Caenorhabditis elegans
Proteomics
Proteome
Proteins
Biological Phenomena
Pheromones
Homeostasis
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

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Methods for Evaluating the Caenorhabditis elegans Dauer State : Standard Dauer-Formation Assay Using Synthetic Daumones and Proteomic Analysis of O-GlcNAc Modifications. / Lee, Jeeyong; Kim, Kwang Youl; Joo, Hyoe Jin; Kim, Heekyeong; Jeong, Pan Young; Paik, Young-Ki.

In: Methods in Cell Biology, Vol. 106, 02.12.2011, p. 445-460.

Research output: Contribution to journalArticle

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