Methods for Evaluating the Caenorhabditis elegans Dauer State: Standard Dauer-Formation Assay Using Synthetic Daumones and Proteomic Analysis of O-GlcNAc Modifications

Jeeyong Lee, Kwang Youl Kim, Hyoe Jin Joo, Heekyeong Kim, Pan Young Jeong, Young Ki Paik

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The dauer state is a non-feeding, alternative L3 state characterized by a number of distinctive metabolic and morphological changes. There are many naturally occurring dauer-inducing pheromones, termed daumones, that have been suggested by some to exhibit differences in dauer-inducing activity. Here, we have established a standard dauer-formation assay that uses synthetic daumones 1, 2, and 3, the three major daumones. To analyze the proteome of Caenorhabditis elegans in the dauer state, we focused on O-GlcNAc modification, a cytosolic modification of proteins that is known to interact either competitively or synergistically with protein phosphorylation. Protein O-GlcNAc modification is an important biological process in cells that can ensure the timely response to extracellular stimuli, such as daumone, and maintain cellular homeostasis. Establishing a standard method for assaying dauer formation using different synthetic daumones, and using differences in O-GlcNAcylated proteins during the dauer state to analyze the dauer proteome will lead to a better understanding of dauer biology of C. elegans in the context of animal longevity and adaptation under harsh environments.

Original languageEnglish
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages445-460
Number of pages16
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Cell Biology
Volume106
ISSN (Print)0091-679X

Bibliographical note

Funding Information:
We thank the Caenorhabditis elegans Genetic Center (CGC) for providing various strains. This work was supported by a WCU (World Class University) program (R31-2008-000-10086-0) and National Research Lab Program (2011-0028112 to YKP) through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (R31-2008-000-10086-0), and a grant from the Korea Health 21 R&D project, Ministry of Health and Welfare of Republic of National Project for Personalized Genome Medicine (A111218-11-CP01 to YKP).

All Science Journal Classification (ASJC) codes

  • Cell Biology

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