The dauer state is a non-feeding, alternative L3 state characterized by a number of distinctive metabolic and morphological changes. There are many naturally occurring dauer-inducing pheromones, termed daumones, that have been suggested by some to exhibit differences in dauer-inducing activity. Here, we have established a standard dauer-formation assay that uses synthetic daumones 1, 2, and 3, the three major daumones. To analyze the proteome of Caenorhabditis elegans in the dauer state, we focused on O-GlcNAc modification, a cytosolic modification of proteins that is known to interact either competitively or synergistically with protein phosphorylation. Protein O-GlcNAc modification is an important biological process in cells that can ensure the timely response to extracellular stimuli, such as daumone, and maintain cellular homeostasis. Establishing a standard method for assaying dauer formation using different synthetic daumones, and using differences in O-GlcNAcylated proteins during the dauer state to analyze the dauer proteome will lead to a better understanding of dauer biology of C. elegans in the context of animal longevity and adaptation under harsh environments.
|Title of host publication||Methods in Cell Biology|
|Publisher||Academic Press Inc.|
|Number of pages||16|
|Publication status||Published - 2011|
|Name||Methods in Cell Biology|
Bibliographical noteFunding Information:
We thank the Caenorhabditis elegans Genetic Center (CGC) for providing various strains. This work was supported by a WCU (World Class University) program (R31-2008-000-10086-0) and National Research Lab Program (2011-0028112 to YKP) through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (R31-2008-000-10086-0), and a grant from the Korea Health 21 R&D project, Ministry of Health and Welfare of Republic of National Project for Personalized Genome Medicine (A111218-11-CP01 to YKP).
All Science Journal Classification (ASJC) codes
- Cell Biology