Methylation of CpG islands in the rat 7-dehydrocholesterol reductase promoter suppresses transcriptional activation

Jai Hyun Kim, Eun Ha Hwang, Hye Jung Park, Young Ki Paik, Yhong Hee Shim

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

In mammals, 7-dehydrocholesterol reductase (Dhcr7) is the terminal enzyme in cholesterol biosynthesis. We previously reported that the Dhcr7 proximal promoter (-179 to +1), which contains CpG islands, is responsible for sterol-mediated expression of the rat gene. In the present study, we examined whether methylation of this region affects the transcriptional activity of the Dhcr7 gene. In vitro DNA methylation of the Dhcr7 promoter and luciferase-reporter assays showed that DNA methylation of the CpG islands suppressed transcription. Furthermore, treatment of the methylated Dhcr7 promoter with the demethylating agent, 5-aza-2′-deoxycytidine (5-Aza-CdR), reversed the suppression of promoter activity. These results indicate that methylation of the CpG islands is an important transcriptional regulatory mechanism in the Dhcr7 promoter.

Original languageEnglish
Pages (from-to)279-282
Number of pages4
JournalMolecules and cells
Volume19
Issue number2
Publication statusPublished - 2005

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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