Methylation status of lamin A/C in gastric cancer cell lines

Won Suk Lee, Jae Joon Jung, Hei Cheul Jeung, Se Won Noh, Bong Kyeong Oh, Ki Yeol Kim, Tae Soo Kim, Hyun Cheol Chung, Jae Kyung Roh, Sun Young Rha

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3 Citations (Scopus)

Abstract

Background/Aims: Epigenetic regulations play a role in the development and progression of cancer. Therefore, discovering novel epigenetically regulated genes could provide useful information in understanding cancer. Lamin A/C is an intermediate filament protein whose expression is reported to be suppressed in tissues of gastro-intestinal malignancies. We examined expression of lamin A/C in gastric and colorectal cancer cell lines and its association with DNA methylation. Methodology: The methylation status of CpG island in 19 gastric, 5 colorectal cancer cells and 1 normal colon cell line were examined with methylation-specific PCR using paired methylated and unmethylated primers. The level of mRNA expression of lamin A/C was detected using RT-PCR. Results: Eighteen gastric cancer cell lines showed 95% unmethylation of lamin A/C and 1 cell line showed partial methylation. In colorectal cancer, only 1 out of 5 cancer cell lines (20%) was partially methylated and the remaining cell lines, including 1 normal colon cell line was unmethylated. With RT-PCR, all cell lines demonstrated mRNA expression of lamin A/C regardless of methylation status. Conclusions: We observed that the expression of lamin A/C was not suppressed in gastrointestinal cancer cell lines different from hematologic malignant cells and it is not regulated through DNA methylation.

Original languageEnglish
Pages (from-to)1313-1318
Number of pages6
JournalHepato-gastroenterology
Volume59
Issue number116
DOIs
Publication statusPublished - 2012 Jun

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

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    Lee, W. S., Jung, J. J., Jeung, H. C., Noh, S. W., Oh, B. K., Kim, K. Y., Kim, T. S., Chung, H. C., Roh, J. K., & Rha, S. Y. (2012). Methylation status of lamin A/C in gastric cancer cell lines. Hepato-gastroenterology, 59(116), 1313-1318. https://doi.org/10.5754/hge11610