Mice with MCH ablation resist diet-induced obesity through strain-specific mechanisms

Efi Kokkotou, Justin Y. Jeon, Xiaomei Wang, Francis E. Marino, Michael Carlson, Daniel J. Trombly, Eleftheria Maratos-Flier

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Genetics and environment contribute to the development of obesity, in both humans, and rodents. However, the potential interaction between genes important in energy balance, strain background, and dietary environment has been only minimally explored. We investigated the effects of genetic ablation of melanin-concentrating hormone (MCH), a neuropeptide with a key role in energy balance, with chow and a high-fat diet (HFD) in two different mouse strains, one obesity-prone (C57BL/6) and the other obesity-resistant (129). Substantial differences were seen in wild-type (WT) animals of different strains. 129 animals had significantly lower levels of spontaneous locomotor activity than C57BL/6; however, 129 mice gained less weight on both chow and HFD. In both strains, deletion of MCH led to attenuated weight gain compared with WT counterparts, an effect secondary to increased energy expenditure. In both strains, feeding a HFD led to further increases in energy expenditure in both WT and MCH-KO mice; however, this increase was more pronounced in 129 mice. In addition, mice lacking MCH have a phenotype of increased locomotor activity, an effect also seen in both strains. The relative increase in activity in MCH -/- mice is modest in animals fed chow but increases substantially when animals are placed on HFD. These studies reinforce the important role of MCH in energy homeostasis and indicate that MCH is a plausible target for antiobesity therapy.

Original languageEnglish
Pages (from-to)R117-R124
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume289
Issue number1 58-1
DOIs
Publication statusPublished - 2005 Jul 1

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Obesity
Diet
High Fat Diet
129 Strain Mouse
Locomotion
Energy Metabolism
Wild Animals
melanin-concentrating hormone
Neuropeptides
Weight Gain
Rodentia
Homeostasis
Phenotype
Weights and Measures
Genes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Kokkotou, Efi ; Jeon, Justin Y. ; Wang, Xiaomei ; Marino, Francis E. ; Carlson, Michael ; Trombly, Daniel J. ; Maratos-Flier, Eleftheria. / Mice with MCH ablation resist diet-induced obesity through strain-specific mechanisms. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2005 ; Vol. 289, No. 1 58-1. pp. R117-R124.
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Mice with MCH ablation resist diet-induced obesity through strain-specific mechanisms. / Kokkotou, Efi; Jeon, Justin Y.; Wang, Xiaomei; Marino, Francis E.; Carlson, Michael; Trombly, Daniel J.; Maratos-Flier, Eleftheria.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 289, No. 1 58-1, 01.07.2005, p. R117-R124.

Research output: Contribution to journalArticle

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