Abstract
Micelle-mediated extraction offers a convenient alternative to conventional extraction systems. A new method based on micellemediated extraction was developed for the separation and determination of dibenzocyclooctadiene lignans from Schisandra chinensis by high-performance liquid chromatography with photodiode array detection. Various experimental conditions using the micelle-mediated method were investigated to evaluate the extraction process. Ethylene glycol monoalkyl ether (Genapol X-080), a non-ionic surfactant oligoethylene glycol monoalkyl ether, was chosen as the extract solvent. The chromatographic separation was accomplished on a Shiseido Capcell Pak C18 analytical column (250 × 4.6mm i.d., 5 mm particle diameter), detected by ultraviolet absorption at 254 nm. The isocratic elution was achieved with a mobile phase composed of water-acetonitrile-formic acid (70:30:0.1) at a flow rate of 0.6 mL/ min. The method was optimized and fully validated against dibenzocyclooctadiene lignans (schizandrin, gomisin A and gomisin N). With 15% Genapol X-080, a liquid to solid ratio of 100:1 (mL/g) and ultrasonic- assisted extraction for 60 min, the extraction percentage of total dibenzocyclooctadiene lignans reached the highest value. The non-ionic surfactant Genapol X-080 solution is an effective alternative for the extraction of bioactive lignans from S. chinensis.
| Original language | English |
|---|---|
| Pages (from-to) | 745-750 |
| Number of pages | 6 |
| Journal | Journal of Chromatographic Science |
| Volume | 52 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2014 Aug |
Fingerprint
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
Cite this
}
Micelle-mediated extraction of dibenzocyclooctadiene lignans from schisandra chinensis with analysis by high-performance liquid chromatography. / Lee, Ki Yong; Shin, Yong Jun; Kim, Dae Hyun; Park, Jin Ho; Kim, Seung Hyun; Han, Sang Beom; Sung, Sang Hyun.
In: Journal of Chromatographic Science, Vol. 52, No. 7, 08.2014, p. 745-750.Research output: Contribution to journal › Article
TY - JOUR
T1 - Micelle-mediated extraction of dibenzocyclooctadiene lignans from schisandra chinensis with analysis by high-performance liquid chromatography
AU - Lee, Ki Yong
AU - Shin, Yong Jun
AU - Kim, Dae Hyun
AU - Park, Jin Ho
AU - Kim, Seung Hyun
AU - Han, Sang Beom
AU - Sung, Sang Hyun
PY - 2014/8
Y1 - 2014/8
N2 - Micelle-mediated extraction offers a convenient alternative to conventional extraction systems. A new method based on micellemediated extraction was developed for the separation and determination of dibenzocyclooctadiene lignans from Schisandra chinensis by high-performance liquid chromatography with photodiode array detection. Various experimental conditions using the micelle-mediated method were investigated to evaluate the extraction process. Ethylene glycol monoalkyl ether (Genapol X-080), a non-ionic surfactant oligoethylene glycol monoalkyl ether, was chosen as the extract solvent. The chromatographic separation was accomplished on a Shiseido Capcell Pak C18 analytical column (250 × 4.6mm i.d., 5 mm particle diameter), detected by ultraviolet absorption at 254 nm. The isocratic elution was achieved with a mobile phase composed of water-acetonitrile-formic acid (70:30:0.1) at a flow rate of 0.6 mL/ min. The method was optimized and fully validated against dibenzocyclooctadiene lignans (schizandrin, gomisin A and gomisin N). With 15% Genapol X-080, a liquid to solid ratio of 100:1 (mL/g) and ultrasonic- assisted extraction for 60 min, the extraction percentage of total dibenzocyclooctadiene lignans reached the highest value. The non-ionic surfactant Genapol X-080 solution is an effective alternative for the extraction of bioactive lignans from S. chinensis.
AB - Micelle-mediated extraction offers a convenient alternative to conventional extraction systems. A new method based on micellemediated extraction was developed for the separation and determination of dibenzocyclooctadiene lignans from Schisandra chinensis by high-performance liquid chromatography with photodiode array detection. Various experimental conditions using the micelle-mediated method were investigated to evaluate the extraction process. Ethylene glycol monoalkyl ether (Genapol X-080), a non-ionic surfactant oligoethylene glycol monoalkyl ether, was chosen as the extract solvent. The chromatographic separation was accomplished on a Shiseido Capcell Pak C18 analytical column (250 × 4.6mm i.d., 5 mm particle diameter), detected by ultraviolet absorption at 254 nm. The isocratic elution was achieved with a mobile phase composed of water-acetonitrile-formic acid (70:30:0.1) at a flow rate of 0.6 mL/ min. The method was optimized and fully validated against dibenzocyclooctadiene lignans (schizandrin, gomisin A and gomisin N). With 15% Genapol X-080, a liquid to solid ratio of 100:1 (mL/g) and ultrasonic- assisted extraction for 60 min, the extraction percentage of total dibenzocyclooctadiene lignans reached the highest value. The non-ionic surfactant Genapol X-080 solution is an effective alternative for the extraction of bioactive lignans from S. chinensis.
UR - http://www.scopus.com/inward/record.url?scp=84904302213&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84904302213&partnerID=8YFLogxK
U2 - 10.1093/chromsci/bmt088
DO - 10.1093/chromsci/bmt088
M3 - Article
C2 - 23828909
AN - SCOPUS:84904302213
VL - 52
SP - 745
EP - 750
JO - Journal of Chromatographic Science
JF - Journal of Chromatographic Science
SN - 0021-9665
IS - 7
ER -