Micelle-mediated extraction of dibenzocyclooctadiene lignans from schisandra chinensis with analysis by high-performance liquid chromatography

Ki Yong Lee, Yong Jun Shin, Dae Hyun Kim, Jin Ho Park, Seung Hyun Kim, Sang Beom Han, Sang Hyun Sung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Micelle-mediated extraction offers a convenient alternative to conventional extraction systems. A new method based on micellemediated extraction was developed for the separation and determination of dibenzocyclooctadiene lignans from Schisandra chinensis by high-performance liquid chromatography with photodiode array detection. Various experimental conditions using the micelle-mediated method were investigated to evaluate the extraction process. Ethylene glycol monoalkyl ether (Genapol X-080), a non-ionic surfactant oligoethylene glycol monoalkyl ether, was chosen as the extract solvent. The chromatographic separation was accomplished on a Shiseido Capcell Pak C18 analytical column (250 × 4.6mm i.d., 5 mm particle diameter), detected by ultraviolet absorption at 254 nm. The isocratic elution was achieved with a mobile phase composed of water-acetonitrile-formic acid (70:30:0.1) at a flow rate of 0.6 mL/ min. The method was optimized and fully validated against dibenzocyclooctadiene lignans (schizandrin, gomisin A and gomisin N). With 15% Genapol X-080, a liquid to solid ratio of 100:1 (mL/g) and ultrasonic- assisted extraction for 60 min, the extraction percentage of total dibenzocyclooctadiene lignans reached the highest value. The non-ionic surfactant Genapol X-080 solution is an effective alternative for the extraction of bioactive lignans from S. chinensis.

Original languageEnglish
Pages (from-to)745-750
Number of pages6
JournalJournal of Chromatographic Science
Volume52
Issue number7
DOIs
Publication statusPublished - 2014 Aug

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High performance liquid chromatography
Micelles
formic acid
Nonionic surfactants
Ether
Lignans
Glycols
Ethylene Glycol
dibenzocyclooctadiene lignan
Photodiodes
Ultrasonics
Flow rate
Water
Liquids
genapol X 080

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Lee, Ki Yong ; Shin, Yong Jun ; Kim, Dae Hyun ; Park, Jin Ho ; Kim, Seung Hyun ; Han, Sang Beom ; Sung, Sang Hyun. / Micelle-mediated extraction of dibenzocyclooctadiene lignans from schisandra chinensis with analysis by high-performance liquid chromatography. In: Journal of Chromatographic Science. 2014 ; Vol. 52, No. 7. pp. 745-750.
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Micelle-mediated extraction of dibenzocyclooctadiene lignans from schisandra chinensis with analysis by high-performance liquid chromatography. / Lee, Ki Yong; Shin, Yong Jun; Kim, Dae Hyun; Park, Jin Ho; Kim, Seung Hyun; Han, Sang Beom; Sung, Sang Hyun.

In: Journal of Chromatographic Science, Vol. 52, No. 7, 08.2014, p. 745-750.

Research output: Contribution to journalArticle

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