Microarray analysis of apple gene expression engaged in early fruit development

Young Pyo Lee, Gyung Hee Yu, Young Sam Seo, Sang Eun Han, Yeon Ok Choi, Daeil Kim, Il Gin Mok, Woo Taek Kim, Soon Kee Sung

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

To evaluate gene expressions mostly engaged in early development of apple fruit, we performed the identification of transcripts differentially expressed in young fruit by using microarrays spotted with 6,253 cDNAs collected from young and mature apple fruits of the cultivar Fuji (Malus domestica Borkh. cv. Fuji). A total of 3,484 cDNAs out of 6,253 were selected after quality control of microarray spots and analyzed for differential gene expression patterns between young fruit and other tissues (mature fruit, leaf and flower). Among them, 192 cDNAs displayed a signal value higher than twofold in young fruit compared with other tissues. Blast analysis of the 192 cDNA clones identified 88 non-redundant groups encoding proteins with known function and 50 non-redundant groups with unknown function. The putative protein products were classified into the following categories: photosynthesis (16.7%), protein synthesis (12.3%), cell proliferation and differentiation (10.9%), cell enlargement (5.8%), metabolism (8.0%), stress response (7.2%), others (2.9%), and unknown functions (32.2%). Furthermore, confirming the microarray data by reverse transcription-polymerase chain reaction revealed that the wide range of transcripts differentially expressed in young fruit was expressed in other organs but not in the mature fruit. The data presented suggested that apple fruit development depends on the tight regulation of the expression of a number of genes, which are also expressed in other organs.

Original languageEnglish
Pages (from-to)917-926
Number of pages10
JournalPlant Cell Reports
Volume26
Issue number7
DOIs
Publication statusPublished - 2007 Jul 1

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fruiting
apples
gene expression
fruits
protein products
Malus domestica
cell differentiation
quality control
early development
cell growth
stress response
cell proliferation
protein synthesis
reverse transcriptase polymerase chain reaction
photosynthesis
clones
flowers
metabolism
cultivars
leaves

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Lee, Y. P., Yu, G. H., Seo, Y. S., Han, S. E., Choi, Y. O., Kim, D., ... Sung, S. K. (2007). Microarray analysis of apple gene expression engaged in early fruit development. Plant Cell Reports, 26(7), 917-926. https://doi.org/10.1007/s00299-007-0308-9
Lee, Young Pyo ; Yu, Gyung Hee ; Seo, Young Sam ; Han, Sang Eun ; Choi, Yeon Ok ; Kim, Daeil ; Mok, Il Gin ; Kim, Woo Taek ; Sung, Soon Kee. / Microarray analysis of apple gene expression engaged in early fruit development. In: Plant Cell Reports. 2007 ; Vol. 26, No. 7. pp. 917-926.
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Lee, YP, Yu, GH, Seo, YS, Han, SE, Choi, YO, Kim, D, Mok, IG, Kim, WT & Sung, SK 2007, 'Microarray analysis of apple gene expression engaged in early fruit development', Plant Cell Reports, vol. 26, no. 7, pp. 917-926. https://doi.org/10.1007/s00299-007-0308-9

Microarray analysis of apple gene expression engaged in early fruit development. / Lee, Young Pyo; Yu, Gyung Hee; Seo, Young Sam; Han, Sang Eun; Choi, Yeon Ok; Kim, Daeil; Mok, Il Gin; Kim, Woo Taek; Sung, Soon Kee.

In: Plant Cell Reports, Vol. 26, No. 7, 01.07.2007, p. 917-926.

Research output: Contribution to journalArticle

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