Abstract
Essential aspects of the innate immune response to microbial infection appear to be conserved between insects and mammals. In order to identify new Drosophila melanogaster genes involved in the immune response, we performed gene expression profiling of Drosophila SL2 cells stimulated with bacterial (LPS/PGN) or fungal (curdlan) components using a cDNA microarray that contained 5,405 Drosophila cDNAs. We found that some genes were similarly regulated by LPS/PGN and curdlan. However, a large number, belonging to the functional classes of cell organization, development, signal transduction, morphogenesis, cell cycle, and DNA replication, displayed significant differences in their transcription profiles between the two treatments, demonstrating that bacterial and fungal components induce different immune response even in an in vitro cell system.
| Original language | English |
|---|---|
| Pages (from-to) | 553-558 |
| Number of pages | 6 |
| Journal | Molecules and Cells |
| Volume | 25 |
| Issue number | 4 |
| Publication status | Published - 2008 Jun 30 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology
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Microarray analysis of the gene expression profiles of SL2 cells stimulated by LPS/PGN and curdlan. / Jin, Li Hua; Choi, Jung Kyoon; Cho, Hwan Sung; Shim, Jaewon; Kim, Young Joon.
In: Molecules and Cells, Vol. 25, No. 4, 30.06.2008, p. 553-558.Research output: Contribution to journal › Article
TY - JOUR
T1 - Microarray analysis of the gene expression profiles of SL2 cells stimulated by LPS/PGN and curdlan
AU - Jin, Li Hua
AU - Choi, Jung Kyoon
AU - Cho, Hwan Sung
AU - Shim, Jaewon
AU - Kim, Young Joon
PY - 2008/6/30
Y1 - 2008/6/30
N2 - Essential aspects of the innate immune response to microbial infection appear to be conserved between insects and mammals. In order to identify new Drosophila melanogaster genes involved in the immune response, we performed gene expression profiling of Drosophila SL2 cells stimulated with bacterial (LPS/PGN) or fungal (curdlan) components using a cDNA microarray that contained 5,405 Drosophila cDNAs. We found that some genes were similarly regulated by LPS/PGN and curdlan. However, a large number, belonging to the functional classes of cell organization, development, signal transduction, morphogenesis, cell cycle, and DNA replication, displayed significant differences in their transcription profiles between the two treatments, demonstrating that bacterial and fungal components induce different immune response even in an in vitro cell system.
AB - Essential aspects of the innate immune response to microbial infection appear to be conserved between insects and mammals. In order to identify new Drosophila melanogaster genes involved in the immune response, we performed gene expression profiling of Drosophila SL2 cells stimulated with bacterial (LPS/PGN) or fungal (curdlan) components using a cDNA microarray that contained 5,405 Drosophila cDNAs. We found that some genes were similarly regulated by LPS/PGN and curdlan. However, a large number, belonging to the functional classes of cell organization, development, signal transduction, morphogenesis, cell cycle, and DNA replication, displayed significant differences in their transcription profiles between the two treatments, demonstrating that bacterial and fungal components induce different immune response even in an in vitro cell system.
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UR - http://www.scopus.com/inward/citedby.url?scp=55449112152&partnerID=8YFLogxK
M3 - Article
C2 - 18460901
AN - SCOPUS:55449112152
VL - 25
SP - 553
EP - 558
JO - Molecules and Cells
JF - Molecules and Cells
SN - 1016-8478
IS - 4
ER -