Microarray based on autodisplayed Ro proteins for medical diagnosis of systemic lupus erythematosus (SLE)

Gu Yoo, Ji Hong Bong, Sinyoung Kim, Joachim Jose, Jae Chul Pyun

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A microarray-based immunoassay for the detection of autoantibodies against Ro protein was developed using Escherichia coli with autodisplayed Ro proteins (Ro(+)-E. coli). Patient serum usually contains various antibodies against the outer membrane components of E. coli as well as autoantibodies against the Ro protein. Therefore, the conventional immunoassay based on Ro(+)-E. coli requires both wild type E. coli (blank test) and Ro(+)-E. coli, and both strains of E. coli must be prepared in situ for each individual test serum. In this study, we tested the feasibility of using several types of animal sera as a replacement for individual human sera. An immunoassay without the blank test was developed using Ro(+)-E. coli by (1) blocking with rabbit serum, and (2) cleaving the Fc region from antibodies using papain. Modified E. coli with autodisplayed Ro protein was immobilized to a surface-modified microplate and the applicability of the immunoassay without the blank test was demonstrated using sera from patients with systemic lupus erythematosus (SLE). Using this approach, a microarray-based fluorescence immunoassay with immobilized Ro(+)-E. coli was able to detect anti-Ro autoantibodies in SLE patient sera with high specificity and selectivity and improved efficiency.

Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalBiosensors and Bioelectronics
Volume57
DOIs
Publication statusPublished - 2014 Jul 15

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Microarrays
Systemic Lupus Erythematosus
Escherichia coli
Proteins
Immunoassay
Serum
Autoantibodies
Antibodies
Immobilized Proteins
Papain
Escherichia coli Proteins
Fluorescence
Animals
Rabbits
Membranes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

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abstract = "A microarray-based immunoassay for the detection of autoantibodies against Ro protein was developed using Escherichia coli with autodisplayed Ro proteins (Ro(+)-E. coli). Patient serum usually contains various antibodies against the outer membrane components of E. coli as well as autoantibodies against the Ro protein. Therefore, the conventional immunoassay based on Ro(+)-E. coli requires both wild type E. coli (blank test) and Ro(+)-E. coli, and both strains of E. coli must be prepared in situ for each individual test serum. In this study, we tested the feasibility of using several types of animal sera as a replacement for individual human sera. An immunoassay without the blank test was developed using Ro(+)-E. coli by (1) blocking with rabbit serum, and (2) cleaving the Fc region from antibodies using papain. Modified E. coli with autodisplayed Ro protein was immobilized to a surface-modified microplate and the applicability of the immunoassay without the blank test was demonstrated using sera from patients with systemic lupus erythematosus (SLE). Using this approach, a microarray-based fluorescence immunoassay with immobilized Ro(+)-E. coli was able to detect anti-Ro autoantibodies in SLE patient sera with high specificity and selectivity and improved efficiency.",
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Microarray based on autodisplayed Ro proteins for medical diagnosis of systemic lupus erythematosus (SLE). / Yoo, Gu; Bong, Ji Hong; Kim, Sinyoung; Jose, Joachim; Pyun, Jae Chul.

In: Biosensors and Bioelectronics, Vol. 57, 15.07.2014, p. 213-218.

Research output: Contribution to journalArticle

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