Microbial acclimatization to lipidic-waste facilitates the efficacy of acidogenic fermentation

Shouvik Saha, Byong Hun Jeon, Mayur B. Kurade, Pradip K. Chatterjee, Soon Woong Chang, Kesavan Markkandan, El Sayed Salama, Sanjay P. Govindwar, Hyunseog Roh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Lipidic-waste such as fat, oil, and grease (FOG) are promising substrates for achieving higher bioenergy yields. An inadequate presence of an effective microbiome in the anaerobic digesters is the bottleneck for the proper utilization of FOG. Gradual introduction of FOG (0.2%, 1.2%, and 2.4% as volatile solids) in acidogenic fermentation showed a significant improvement in hydrogen yield (72%), compared to the control, after 2.4% FOG loading. Volatile solid (VS) reduction reached up to 65% in high FOG reactors with complete removal of major unsaturated fatty acids. Removal of saturated fatty acids increased to 90%. Improvement in hydrogen productivity (46 mL d−1) occurred during step-wise loading of 2.4% FOG to the acclimatized microbiome. The metabolic shift toward carboxylic chain elongation produced C4 and C6 fatty acids at concentrations of 1.61 mM and 0.90 mM, respectively in the acidogenic reactors. High-throughput sequencing of 16S rRNA amplicons revealed that the acclimatization process enriched the phylum Firmicutes (90%), followed by Bacteroidetes (12%) and Cloacimonetes (11%). The abundance of these phyla and their respective genera confirmed their preeminent role in hydrolysis, hydrogenogenic acidogenesis, and carboxylic chain elongation to produce hydrogen and C4–C7 fatty acids. Thus, we suggest that the improvement of hydrogen production using a microbiome acclimatized to FOG, and simultaneous production of high value organics (C4–C7 fatty acids), could facilitate the greater efficacy of the acidogenic fermentation.

Original languageEnglish
Pages (from-to)188-196
Number of pages9
JournalChemical Engineering Journal
Volume358
DOIs
Publication statusPublished - 2019 Feb 15

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Lubricating greases
Oils and fats
acclimation
Fermentation
fermentation
fat
oil
fatty acid
Fatty Acids
Fatty acids
hydrogen
Hydrogen
Elongation
Saturated fatty acids
Unsaturated fatty acids
Organic acids
bioenergy
Hydrogen production
Unsaturated Fatty Acids
organic acid

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Saha, Shouvik ; Jeon, Byong Hun ; Kurade, Mayur B. ; Chatterjee, Pradip K. ; Chang, Soon Woong ; Markkandan, Kesavan ; Salama, El Sayed ; Govindwar, Sanjay P. ; Roh, Hyunseog. / Microbial acclimatization to lipidic-waste facilitates the efficacy of acidogenic fermentation. In: Chemical Engineering Journal. 2019 ; Vol. 358. pp. 188-196.
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abstract = "Lipidic-waste such as fat, oil, and grease (FOG) are promising substrates for achieving higher bioenergy yields. An inadequate presence of an effective microbiome in the anaerobic digesters is the bottleneck for the proper utilization of FOG. Gradual introduction of FOG (0.2{\%}, 1.2{\%}, and 2.4{\%} as volatile solids) in acidogenic fermentation showed a significant improvement in hydrogen yield (72{\%}), compared to the control, after 2.4{\%} FOG loading. Volatile solid (VS) reduction reached up to 65{\%} in high FOG reactors with complete removal of major unsaturated fatty acids. Removal of saturated fatty acids increased to 90{\%}. Improvement in hydrogen productivity (46 mL d−1) occurred during step-wise loading of 2.4{\%} FOG to the acclimatized microbiome. The metabolic shift toward carboxylic chain elongation produced C4 and C6 fatty acids at concentrations of 1.61 mM and 0.90 mM, respectively in the acidogenic reactors. High-throughput sequencing of 16S rRNA amplicons revealed that the acclimatization process enriched the phylum Firmicutes (90{\%}), followed by Bacteroidetes (12{\%}) and Cloacimonetes (11{\%}). The abundance of these phyla and their respective genera confirmed their preeminent role in hydrolysis, hydrogenogenic acidogenesis, and carboxylic chain elongation to produce hydrogen and C4–C7 fatty acids. Thus, we suggest that the improvement of hydrogen production using a microbiome acclimatized to FOG, and simultaneous production of high value organics (C4–C7 fatty acids), could facilitate the greater efficacy of the acidogenic fermentation.",
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Saha, S, Jeon, BH, Kurade, MB, Chatterjee, PK, Chang, SW, Markkandan, K, Salama, ES, Govindwar, SP & Roh, H 2019, 'Microbial acclimatization to lipidic-waste facilitates the efficacy of acidogenic fermentation', Chemical Engineering Journal, vol. 358, pp. 188-196. https://doi.org/10.1016/j.cej.2018.09.220

Microbial acclimatization to lipidic-waste facilitates the efficacy of acidogenic fermentation. / Saha, Shouvik; Jeon, Byong Hun; Kurade, Mayur B.; Chatterjee, Pradip K.; Chang, Soon Woong; Markkandan, Kesavan; Salama, El Sayed; Govindwar, Sanjay P.; Roh, Hyunseog.

In: Chemical Engineering Journal, Vol. 358, 15.02.2019, p. 188-196.

Research output: Contribution to journalArticle

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