Microbial analysis in primary and persistent endodontic infections by using pyrosequencing

Bo Young Hong, Tae Kwon Lee, Sang Min Lim, Seok Woo Chang, Joonhong Park, Seung Hyun Han, Qiang Zhu, Kamran E. Safavi, Ashraf F. Fouad, Kee Yeon Kum

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Introduction The aim of this study was to investigate the bacterial community profile of intracanal microbiota in primary and persistent endodontic infections associated with asymptomatic chronic apical periodontitis by using GS-FLX Titanium pyrosequencing. The null hypothesis was that there is no difference in diversity of overall bacterial community profiles between primary and persistent infections. Methods Pyrosequencing analysis from 10 untreated and 8 root-filled samples was conducted. Results Analysis from 18 samples yielded total of 124,767 16S rRNA gene sequences (with a mean of 6932 reads per sample) that were taxonomically assigned into 803 operational taxonomic units (3% distinction), 148 genera, and 10 phyla including unclassified. Bacteroidetes was the most abundant phylum in both primary and persistent infections. There were no significant differences in bacterial diversity between the 2 infection groups (P >.05). The bacterial community profile that was based on dendrogram showed that bacterial population in both infections was not significantly different in their structure and composition (P >.05). Conclusions The present pyrosequencing study demonstrates that persistent infections have as diverse bacterial community as primary infections.

Original languageEnglish
Pages (from-to)1136-1140
Number of pages5
JournalJournal of Endodontics
Volume39
Issue number9
DOIs
Publication statusPublished - 2013 Sep 1

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Endodontics
Infection
Periapical Periodontitis
Bacteroidetes
Chronic Periodontitis
Microbiota
Titanium
rRNA Genes
Population

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Hong, Bo Young ; Lee, Tae Kwon ; Lim, Sang Min ; Chang, Seok Woo ; Park, Joonhong ; Han, Seung Hyun ; Zhu, Qiang ; Safavi, Kamran E. ; Fouad, Ashraf F. ; Kum, Kee Yeon. / Microbial analysis in primary and persistent endodontic infections by using pyrosequencing. In: Journal of Endodontics. 2013 ; Vol. 39, No. 9. pp. 1136-1140.
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Hong, BY, Lee, TK, Lim, SM, Chang, SW, Park, J, Han, SH, Zhu, Q, Safavi, KE, Fouad, AF & Kum, KY 2013, 'Microbial analysis in primary and persistent endodontic infections by using pyrosequencing', Journal of Endodontics, vol. 39, no. 9, pp. 1136-1140. https://doi.org/10.1016/j.joen.2013.05.001

Microbial analysis in primary and persistent endodontic infections by using pyrosequencing. / Hong, Bo Young; Lee, Tae Kwon; Lim, Sang Min; Chang, Seok Woo; Park, Joonhong; Han, Seung Hyun; Zhu, Qiang; Safavi, Kamran E.; Fouad, Ashraf F.; Kum, Kee Yeon.

In: Journal of Endodontics, Vol. 39, No. 9, 01.09.2013, p. 1136-1140.

Research output: Contribution to journalArticle

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AU - Hong, Bo Young

AU - Lee, Tae Kwon

AU - Lim, Sang Min

AU - Chang, Seok Woo

AU - Park, Joonhong

AU - Han, Seung Hyun

AU - Zhu, Qiang

AU - Safavi, Kamran E.

AU - Fouad, Ashraf F.

AU - Kum, Kee Yeon

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Introduction The aim of this study was to investigate the bacterial community profile of intracanal microbiota in primary and persistent endodontic infections associated with asymptomatic chronic apical periodontitis by using GS-FLX Titanium pyrosequencing. The null hypothesis was that there is no difference in diversity of overall bacterial community profiles between primary and persistent infections. Methods Pyrosequencing analysis from 10 untreated and 8 root-filled samples was conducted. Results Analysis from 18 samples yielded total of 124,767 16S rRNA gene sequences (with a mean of 6932 reads per sample) that were taxonomically assigned into 803 operational taxonomic units (3% distinction), 148 genera, and 10 phyla including unclassified. Bacteroidetes was the most abundant phylum in both primary and persistent infections. There were no significant differences in bacterial diversity between the 2 infection groups (P >.05). The bacterial community profile that was based on dendrogram showed that bacterial population in both infections was not significantly different in their structure and composition (P >.05). Conclusions The present pyrosequencing study demonstrates that persistent infections have as diverse bacterial community as primary infections.

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