Abstract
Crops lack genetic resistance to most necrotrophic pathogens. To compensate for this disadvantage, plants recruit antagonistic members of the soil microbiome to defend their roots against pathogens and other pests. The best examples of this microbially based defense of roots are observed in disease-suppressive soils in which suppressiveness is induced by continuously growing crops that are susceptible to a pathogen, but the molecular basis of most is poorly understood. Here we report the microbial characterization of a Korean soil with specific suppressiveness to Fusarium wilt of strawberry. In this soil, an attack on strawberry roots by Fusarium oxysporum results in a response by microbial defenders, of which members of the Actinobacteria appear to have a key role. We also identify Streptomyces genes responsible for the ribosomal synthesis of a novel heat-stable antifungal thiopeptide antibiotic inhibitory to F. oxysporum and the antibiotic's mode of action against fungal cell wall biosynthesis. Both classical-and community-oriented approaches were required to dissect this suppressive soil from the field to the molecular level, and the results highlight the role of natural antibiotics as weapons in the microbial warfare in the rhizosphere that is integral to plant health, vigor and development.
| Original language | English |
|---|---|
| Pages (from-to) | 119-129 |
| Number of pages | 11 |
| Journal | ISME Journal |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2016 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Ecology, Evolution, Behavior and Systematics
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Microbial and biochemical basis of a Fusarium wilt-suppressive soil. / Cha, Jae Yul; Han, Sangjo; Hong, Hee Jeon; Cho, Hyunji; Kim, Daran; Kwon, Youngho; Kwon, Soon Kyeong; Crusemann, Max; Bok Lee, Yong; Kim, Jihyun F.; Giaever, Guri; Nislow, Corey; Moore, Bradley S.; Thomashow, Linda S.; Weller, David M.; Kwak, Youn Sig.
In: ISME Journal, Vol. 10, No. 1, 01.01.2016, p. 119-129.Research output: Contribution to journal › Article
TY - JOUR
T1 - Microbial and biochemical basis of a Fusarium wilt-suppressive soil
AU - Cha, Jae Yul
AU - Han, Sangjo
AU - Hong, Hee Jeon
AU - Cho, Hyunji
AU - Kim, Daran
AU - Kwon, Youngho
AU - Kwon, Soon Kyeong
AU - Crusemann, Max
AU - Bok Lee, Yong
AU - Kim, Jihyun F.
AU - Giaever, Guri
AU - Nislow, Corey
AU - Moore, Bradley S.
AU - Thomashow, Linda S.
AU - Weller, David M.
AU - Kwak, Youn Sig
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Crops lack genetic resistance to most necrotrophic pathogens. To compensate for this disadvantage, plants recruit antagonistic members of the soil microbiome to defend their roots against pathogens and other pests. The best examples of this microbially based defense of roots are observed in disease-suppressive soils in which suppressiveness is induced by continuously growing crops that are susceptible to a pathogen, but the molecular basis of most is poorly understood. Here we report the microbial characterization of a Korean soil with specific suppressiveness to Fusarium wilt of strawberry. In this soil, an attack on strawberry roots by Fusarium oxysporum results in a response by microbial defenders, of which members of the Actinobacteria appear to have a key role. We also identify Streptomyces genes responsible for the ribosomal synthesis of a novel heat-stable antifungal thiopeptide antibiotic inhibitory to F. oxysporum and the antibiotic's mode of action against fungal cell wall biosynthesis. Both classical-and community-oriented approaches were required to dissect this suppressive soil from the field to the molecular level, and the results highlight the role of natural antibiotics as weapons in the microbial warfare in the rhizosphere that is integral to plant health, vigor and development.
AB - Crops lack genetic resistance to most necrotrophic pathogens. To compensate for this disadvantage, plants recruit antagonistic members of the soil microbiome to defend their roots against pathogens and other pests. The best examples of this microbially based defense of roots are observed in disease-suppressive soils in which suppressiveness is induced by continuously growing crops that are susceptible to a pathogen, but the molecular basis of most is poorly understood. Here we report the microbial characterization of a Korean soil with specific suppressiveness to Fusarium wilt of strawberry. In this soil, an attack on strawberry roots by Fusarium oxysporum results in a response by microbial defenders, of which members of the Actinobacteria appear to have a key role. We also identify Streptomyces genes responsible for the ribosomal synthesis of a novel heat-stable antifungal thiopeptide antibiotic inhibitory to F. oxysporum and the antibiotic's mode of action against fungal cell wall biosynthesis. Both classical-and community-oriented approaches were required to dissect this suppressive soil from the field to the molecular level, and the results highlight the role of natural antibiotics as weapons in the microbial warfare in the rhizosphere that is integral to plant health, vigor and development.
UR - http://www.scopus.com/inward/record.url?scp=84930799307&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84930799307&partnerID=8YFLogxK
U2 - 10.1038/ismej.2015.95
DO - 10.1038/ismej.2015.95
M3 - Article
C2 - 26057845
AN - SCOPUS:84930799307
VL - 10
SP - 119
EP - 129
JO - ISME Journal
JF - ISME Journal
SN - 1751-7362
IS - 1
ER -