The methane productivity and long chain fatty acids (LCFAs) degradation capability of unacclimatized seed sludge (USS) and acclimatized seed sludge (ASS) at different substrate ratios of fats oil and grease (FOG) and mixed sewage sludge were investigated in this study. Biogas produced in ASS in initial phase of anaerobic digestion had higher methane content (65–76%) than that in USS (26–73%). The degradation of major LCFAs in the ASS was 22–80%, 33–191%, and 7–64% higher for the substrate ratios of 100:10, 100:20, and 100:30, respectively, as compared to the LCFAs’ degradation in USS. Microbial acclimatization increased the population of Firmicutes (40%), Bacteroidetes (32%), Synergistetes (10%), and Euryarchaeota (8%) in ASS, which supported the faster rate of LCFAs degradation for its later conversion to methane. The significant abundance of Syntrophomonas and Methanosarcina genera in ASS supported faster generation rate of methane in an obligatory syntrophic relationship.
Bibliographical noteFunding Information:
This study was supported by the National Research Foundation of Korea (NRF), Ministry of Education, Science, and Technology ( MEST ) of the South Korean government (NRF- No. 2019R1I1A1A01063318 ). Authors also thankfully acknowledge Korea Institute of Energy Technology Evaluation and Planning (KETEP), and the Ministry of Trade, Industry and Energy ( MOTIE ) of the Republic of Korea (No. 20173010092470 ).
All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal