Microbiome and mycobiome interaction in house dust mites and impact on airway cells

Ju Yeong Kim, Myung hee Yi, Seogwon Lee, In Yong Lee, Dongeun Yong, Sang Sun Yoon, Tai Soon Yong

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Background: Major allergen sources Dermatophagoides farinae, Dermatophagoides pteronyssinus and Tyrophagus putrescentiae have been reported to have distinct microbiomes. The purpose of this study was to investigate the effect of each mite on airway epithelial cells as a model of airway allergic disease. Methods: Transcriptomic analysis (RNA-seq) of an airway epithelial cell line (BEAS-2B) was performed to compare gene expression patterns after treatment with extracts of three mite species (D. farinae, D. pteronyssinus and T. putrescentiae). In addition, mycobiome deep sequencing of mites was employed to identify fungal species that interact with the microbiomes of the mites. Results: Immune responses to bacteria were enriched only in the D. farinae-treated group as this species harboured larger numbers of bacteria than the other mites, and the high level of LPS in D. farinae caused proinflammatory cytokine production in airway epithelial cells. In addition, antibiotic metabolism pathways were enriched in D. pteronyssinus-treated cells but not in D. farinae -treated cells. Subsequent experiments revealed that D. pteronyssinus had a high fungal load that inhibited bacterial survival in this mite species. Conclusion: The large amount of bacteria in D. farinae causes airway epithelial cells to produce more allergy-related cytokines than D. pteronyssinus, since fungi present in D. pteronyssinus suppress the abundance of mite-associated bacteria.

Original languageEnglish
Pages (from-to)1592-1602
Number of pages11
JournalClinical and Experimental Allergy
Issue number12
Publication statusPublished - 2021 Dec

Bibliographical note

Funding Information:
This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (Ministry of Education, Science and Technology; grant no. 2019R1A2B5B01069843 and no. 2020R1I1A2074562) and by the Ministry of Health and Welfare, Republic of Korea (grant no. HI14C1324).

Publisher Copyright:
© 2021 John Wiley & Sons Ltd

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology


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