TY - JOUR
T1 - Microchip electrophoresis with wall-jet electrochemical detector
T2 - Influence of detection potential upon resolution of solutes
AU - Pumera, Martin
AU - Merkoĉo, Arben
AU - Alegret, Salvador
PY - 2006/12
Y1 - 2006/12
N2 - This report studies the electrochemical response of wall-jet detector for microchip electrophoresis (μCE). It shows that in wall-jet configuration, the electrochemical detector operates in coulometric mode and that there is an influence of detection potential upon peak width and therefore upon the resolution of solutes. Upon raising the detection potential from +0.3 to +0.9 V, the resolution between model analytes, dopamine and catechol, increases from 0.63 to 2.90. The reasons for this behavior originate in wall-jet detector design and in its typically significant higher detector volume than the volume of injected sample. The conversion efficiency of the wall-jet electrochemical detection cell was found to be 97.4% for dopamine and 98.0% for catechol. The paper brings deeper understanding of operations of wall-jet electrochemical detectors for microchip devices, and it explains previously reported significantly sharper peaks when electrocatalytic electrodes (i.e., palladium and carbon nanotube) were used in μCE-electrochemistry wall-jet detector.
AB - This report studies the electrochemical response of wall-jet detector for microchip electrophoresis (μCE). It shows that in wall-jet configuration, the electrochemical detector operates in coulometric mode and that there is an influence of detection potential upon peak width and therefore upon the resolution of solutes. Upon raising the detection potential from +0.3 to +0.9 V, the resolution between model analytes, dopamine and catechol, increases from 0.63 to 2.90. The reasons for this behavior originate in wall-jet detector design and in its typically significant higher detector volume than the volume of injected sample. The conversion efficiency of the wall-jet electrochemical detection cell was found to be 97.4% for dopamine and 98.0% for catechol. The paper brings deeper understanding of operations of wall-jet electrochemical detectors for microchip devices, and it explains previously reported significantly sharper peaks when electrocatalytic electrodes (i.e., palladium and carbon nanotube) were used in μCE-electrochemistry wall-jet detector.
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U2 - 10.1002/elps.200600386
DO - 10.1002/elps.200600386
M3 - Article
C2 - 17117390
AN - SCOPUS:33846008001
VL - 27
SP - 5068
EP - 5072
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 24
ER -