Microchip separation and electrochemical detection of amino acids and peptides following precolumn derivatization with naphthalene-2,3-dicarboxyaldehyde

Joseph Wang, Gang Chen, Martin Pumera

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

An integrated microfluidic device is used to derivatize, separate, and amperometrically detect amino acids and peptides in the presence of naphthalene-2,3-dicarboxyaldehyde (NDA). The integrated system offers a rapid (4 min) simultaneous measurements of 5 amino acids (Arg, Lys, Gly, Cys, PhenA) down to the 3.2 μM level in connection to a precolumn reaction chamber, an electrophoretic separation channel, and an end-column thick-film carbon-electrode detector. The effect of the separation voltage, detection potential, reagent concentration, and other variables on the response is examined. Calibration and precision experiments indicate a linear and reproducible response. Applicability for the separation and detection of small peptides is demonstrated. Such on-chip generation of electroactive products offers great promise for detecting other nonelectroactive analytes.

Original languageEnglish
Pages (from-to)862-865
Number of pages4
JournalElectroanalysis
Volume15
Issue number10
DOIs
Publication statusPublished - 2003 Jul 1

Fingerprint

Naphthalene
Peptides
Amino acids
Amino Acids
Thick films
Microfluidics
Carbon
Calibration
Detectors
Electrodes
Electric potential
naphthalene
Experiments

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Electrochemistry

Cite this

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abstract = "An integrated microfluidic device is used to derivatize, separate, and amperometrically detect amino acids and peptides in the presence of naphthalene-2,3-dicarboxyaldehyde (NDA). The integrated system offers a rapid (4 min) simultaneous measurements of 5 amino acids (Arg, Lys, Gly, Cys, PhenA) down to the 3.2 μM level in connection to a precolumn reaction chamber, an electrophoretic separation channel, and an end-column thick-film carbon-electrode detector. The effect of the separation voltage, detection potential, reagent concentration, and other variables on the response is examined. Calibration and precision experiments indicate a linear and reproducible response. Applicability for the separation and detection of small peptides is demonstrated. Such on-chip generation of electroactive products offers great promise for detecting other nonelectroactive analytes.",
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Microchip separation and electrochemical detection of amino acids and peptides following precolumn derivatization with naphthalene-2,3-dicarboxyaldehyde. / Wang, Joseph; Chen, Gang; Pumera, Martin.

In: Electroanalysis, Vol. 15, No. 10, 01.07.2003, p. 862-865.

Research output: Contribution to journalArticle

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