Abstract
An integrated microfluidic device is used to derivatize, separate, and amperometrically detect amino acids and peptides in the presence of naphthalene-2,3-dicarboxyaldehyde (NDA). The integrated system offers a rapid (4 min) simultaneous measurements of 5 amino acids (Arg, Lys, Gly, Cys, PhenA) down to the 3.2 μM level in connection to a precolumn reaction chamber, an electrophoretic separation channel, and an end-column thick-film carbon-electrode detector. The effect of the separation voltage, detection potential, reagent concentration, and other variables on the response is examined. Calibration and precision experiments indicate a linear and reproducible response. Applicability for the separation and detection of small peptides is demonstrated. Such on-chip generation of electroactive products offers great promise for detecting other nonelectroactive analytes.
| Original language | English |
|---|---|
| Pages (from-to) | 862-865 |
| Number of pages | 4 |
| Journal | Electroanalysis |
| Volume | 15 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2003 Jul 1 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Electrochemistry
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Microchip separation and electrochemical detection of amino acids and peptides following precolumn derivatization with naphthalene-2,3-dicarboxyaldehyde. / Wang, Joseph; Chen, Gang; Pumera, Martin.
In: Electroanalysis, Vol. 15, No. 10, 01.07.2003, p. 862-865.Research output: Contribution to journal › Article
TY - JOUR
T1 - Microchip separation and electrochemical detection of amino acids and peptides following precolumn derivatization with naphthalene-2,3-dicarboxyaldehyde
AU - Wang, Joseph
AU - Chen, Gang
AU - Pumera, Martin
PY - 2003/7/1
Y1 - 2003/7/1
N2 - An integrated microfluidic device is used to derivatize, separate, and amperometrically detect amino acids and peptides in the presence of naphthalene-2,3-dicarboxyaldehyde (NDA). The integrated system offers a rapid (4 min) simultaneous measurements of 5 amino acids (Arg, Lys, Gly, Cys, PhenA) down to the 3.2 μM level in connection to a precolumn reaction chamber, an electrophoretic separation channel, and an end-column thick-film carbon-electrode detector. The effect of the separation voltage, detection potential, reagent concentration, and other variables on the response is examined. Calibration and precision experiments indicate a linear and reproducible response. Applicability for the separation and detection of small peptides is demonstrated. Such on-chip generation of electroactive products offers great promise for detecting other nonelectroactive analytes.
AB - An integrated microfluidic device is used to derivatize, separate, and amperometrically detect amino acids and peptides in the presence of naphthalene-2,3-dicarboxyaldehyde (NDA). The integrated system offers a rapid (4 min) simultaneous measurements of 5 amino acids (Arg, Lys, Gly, Cys, PhenA) down to the 3.2 μM level in connection to a precolumn reaction chamber, an electrophoretic separation channel, and an end-column thick-film carbon-electrode detector. The effect of the separation voltage, detection potential, reagent concentration, and other variables on the response is examined. Calibration and precision experiments indicate a linear and reproducible response. Applicability for the separation and detection of small peptides is demonstrated. Such on-chip generation of electroactive products offers great promise for detecting other nonelectroactive analytes.
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U2 - 10.1002/elan.200390106
DO - 10.1002/elan.200390106
M3 - Article
AN - SCOPUS:0041767342
VL - 15
SP - 862
EP - 865
JO - Electroanalysis
JF - Electroanalysis
SN - 1040-0397
IS - 10
ER -