MicroDuMIP: Target-enrichment technique for microarray-based duplex Molecular inversion probes

Jung Ki Yoon, Jinwoo Ahn, Han Sang Kim, Soo Min Han, Hoon Jang, Min Goo Lee, Ji Hyun Lee, Duhee Bang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Molecular inversion probe (MIP)-based capture is a scalable and effective target-enrichment technology that can use synthetic single-stranded oligonucleotides as probes. Unlike the straightforward use of synthetic oligonucleotides for low-throughput target capture, high-throughput MIP capture has required laborious protocols to generate thousands of singlestranded probes from DNA microarray because of multiple enzymatic steps, gel purifications and extensive PCR amplifications. Here, we developed a simple and efficient microarray-based MIP preparation protocol using only one enzyme with doublestranded probes and improved target capture yields by designing probes with overlapping targets and unique barcodes. To test our strategy, we produced 11 510 microarray-based duplex MIPs (microDuMIPs) and captured 3554 exons of 228 genes in a HapMap genomic DNA sample (NA12878). Under our protocol, capture performance and precision of calling were compatible to conventional MIP capture methods, yet overlapping targets and unique barcodes allowed us to precisely genotype with as little as 50 ng of input genomic DNA without library preparation. microDu-MIPmethod is simpler and cheaper, allowing broader applications and accurate target sequencing with a scalable number of targets.

Original languageEnglish
Article numbere28
JournalNucleic acids research
Volume43
Issue number5
DOIs
Publication statusPublished - 2015 Mar 11

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint Dive into the research topics of 'MicroDuMIP: Target-enrichment technique for microarray-based duplex Molecular inversion probes'. Together they form a unique fingerprint.

  • Cite this