Microfabricated conductometric urea biosensor based on sol-gel immobilized urease

Won Yong Lee, Kang Shin Lee, Tae Han Kim, Min Chol Shin, Je Kyun Park

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A conductometric biosensor was constructed with a sol-gel immobilized urease on a microfabricated interdigitated array (IDA) gold electrode. Tetramethoxysilane (TMOS) was used as a sol-gel precursor for the immobilization of the urease and the performance of the resulting urea biosensor was controlled through the water content in the acid-catalyzed hydrolysis of the sol-gel stock solution. The sol-gel-derived urea biosensor showed relatively wide dynamic range of 0.2-50 mM in 5 mM imidazole-HC1 buffer at pH 7.5. Furthermore, the urea biosensor exhibited good sensor-to-sensor reproducibility (6.5 %) and storage stability (50 % of its original activity retained after 3 weeks) when stored in 5 mM imidazole-HC1 buffer at 4 °C. The urea biosensor was successfully applied for the urea determination in urine sample by employing the differential measurement format consisting of an active IDA conductometric sensor containing sol-gel-immobilized urease and a reference IDA conductometric sensor containing bovine serum albumin layer instead of the urease.

Original languageEnglish
Pages (from-to)78-82
Number of pages5
JournalElectroanalysis
Volume12
Issue number1
DOIs
Publication statusPublished - 2000 Dec 14

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Urease
Biosensors
Urea
Sol-gels
Sensor arrays
Buffers
Sensors
Bovine Serum Albumin
Gold
Water content
Hydrolysis
Electrodes
Acids

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Electrochemistry

Cite this

Lee, Won Yong ; Lee, Kang Shin ; Kim, Tae Han ; Shin, Min Chol ; Park, Je Kyun. / Microfabricated conductometric urea biosensor based on sol-gel immobilized urease. In: Electroanalysis. 2000 ; Vol. 12, No. 1. pp. 78-82.
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Microfabricated conductometric urea biosensor based on sol-gel immobilized urease. / Lee, Won Yong; Lee, Kang Shin; Kim, Tae Han; Shin, Min Chol; Park, Je Kyun.

In: Electroanalysis, Vol. 12, No. 1, 14.12.2000, p. 78-82.

Research output: Contribution to journalArticle

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