Microfabricated protein-containing poly(ethylene glycol) hydrogel arrays for biosensing

Vamsi K. Yadavalli, Won Gun Koh, George J. Lazur, Michael V. Pishko

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

We describe the use of poly(ethylene glycol) (PEG) hydrogel microstructures containing immobilized enzymes such as acetylcholinesterase (AChE) and urease for the detection of analytes. Changes in the microenvironment pH due to enzyme-catalyzed reaction with the analyte of interest were used to fluorescently detect low concentrations of the analyte. The hydrogel microstructures were prepared photolithographically on silicon surfaces. The use of a microarraying robot to create microstructured hydrogel array sensors is also described. A dual analyte sensor using this scheme is presented to demonstrate the ease of creation of such multianalyte sensors. Such micron-sized arrays can therefore be easily used as biosensors to detect small molecular weight analytes to extremely low concentrations in air or water.

Original languageEnglish
Pages (from-to)290-297
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume97
Issue number2-3
DOIs
Publication statusPublished - 2004 Feb 1

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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