We describe the use of poly(ethylene glycol) (PEG) hydrogel microstructures containing immobilized enzymes such as acetylcholinesterase (AChE) and urease for the detection of analytes. Changes in the microenvironment pH due to enzyme-catalyzed reaction with the analyte of interest were used to fluorescently detect low concentrations of the analyte. The hydrogel microstructures were prepared photolithographically on silicon surfaces. The use of a microarraying robot to create microstructured hydrogel array sensors is also described. A dual analyte sensor using this scheme is presented to demonstrate the ease of creation of such multianalyte sensors. Such micron-sized arrays can therefore be easily used as biosensors to detect small molecular weight analytes to extremely low concentrations in air or water.
Bibliographical noteFunding Information:
We gratefully acknowledge financial support from the National Aeronautics and Space Administration (NAG 9 1277) and also the assistance of Craig Praul in the DNA Microarray facility of Penn State’s Huck Institutes of the Life Sciences in fabricating our hydrogel microarrays.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry