Microfluidic array chip for single-cell isolation using two-way pneumatic actuation

Young Ji Kim, Jaehoon Chung, Hyung Kew Lee, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

In this work, we have implemented a new microfluidic chip which can capture and isolate single-cells in a microchamber array for various single-cell assays. Proposed microfluidic chip consists of two PDMS (Polydimethylsiloxane) layers: a microfluidic channel layer for injecting cells and reagents and an air channel for pneumatic actuation. Each microfluidic chip has 27 microchambers for single-cell culture and analysis. In order to verify our single-cell capturing scheme, we tested the fabricated chip with 15?m-diameter polystyrene microbeads. We successfully trapped the microbeads in each the capture site with a capture rate higher than 80% and loaded them into the corresponding chambers. After loading, the chambers were isolated from one another by pneumatic actuation. The isolation was confirmed by flowing blue ink into the main flow channel.

Original languageEnglish
Title of host publicationMEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems
Pages14-17
Number of pages4
DOIs
Publication statusPublished - 2008 Aug 29
Event21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson - Tucson, AZ, United States
Duration: 2008 Jan 132008 Jan 17

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson
CountryUnited States
CityTucson, AZ
Period08/1/1308/1/17

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Microfluidic array chip for single-cell isolation using two-way pneumatic actuation'. Together they form a unique fingerprint.

  • Cite this

    Kim, Y. J., Chung, J., Lee, H. K., & Yoon, E. (2008). Microfluidic array chip for single-cell isolation using two-way pneumatic actuation. In MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems (pp. 14-17). [4443581] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2008.4443581