Microfluidic array chip for single-cell isolation using two-way pneumatic actuation

Young Ji Kim; Jaehoon Chung; Hyung Kew Lee; Euisik Yoon

doi:10.1109/MEMSYS.2008.4443581

Microfluidic array chip for single-cell isolation using two-way pneumatic actuation

Young Ji Kim, Jaehoon Chung, Hyung Kew Lee, Euisik Yoon

Yonsei Advanced Science Institute

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

5 Citations (Scopus)

Abstract

In this work, we have implemented a new microfluidic chip which can capture and isolate single-cells in a microchamber array for various single-cell assays. Proposed microfluidic chip consists of two PDMS (Polydimethylsiloxane) layers: a microfluidic channel layer for injecting cells and reagents and an air channel for pneumatic actuation. Each microfluidic chip has 27 microchambers for single-cell culture and analysis. In order to verify our single-cell capturing scheme, we tested the fabricated chip with 15?m-diameter polystyrene microbeads. We successfully trapped the microbeads in each the capture site with a capture rate higher than 80% and loaded them into the corresponding chambers. After loading, the chambers were isolated from one another by pneumatic actuation. The isolation was confirmed by flowing blue ink into the main flow channel.

Original language	English
Title of host publication	MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems
Pages	14-17
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2008.4443581
Publication status	Published - 2008
Event	21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson - Tucson, AZ, United States Duration: 2008 Jan 13 → 2008 Jan 17

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson
Country/Territory	United States
City	Tucson, AZ
Period	08/1/13 → 08/1/17

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2008.4443581

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Kim, Y. J., Chung, J., Lee, H. K., & Yoon, E. (2008). Microfluidic array chip for single-cell isolation using two-way pneumatic actuation. In MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems (pp. 14-17). [4443581] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2008.4443581

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title = "Microfluidic array chip for single-cell isolation using two-way pneumatic actuation",

abstract = "In this work, we have implemented a new microfluidic chip which can capture and isolate single-cells in a microchamber array for various single-cell assays. Proposed microfluidic chip consists of two PDMS (Polydimethylsiloxane) layers: a microfluidic channel layer for injecting cells and reagents and an air channel for pneumatic actuation. Each microfluidic chip has 27 microchambers for single-cell culture and analysis. In order to verify our single-cell capturing scheme, we tested the fabricated chip with 15?m-diameter polystyrene microbeads. We successfully trapped the microbeads in each the capture site with a capture rate higher than 80% and loaded them into the corresponding chambers. After loading, the chambers were isolated from one another by pneumatic actuation. The isolation was confirmed by flowing blue ink into the main flow channel.",

author = "Kim, {Young Ji} and Jaehoon Chung and Lee, {Hyung Kew} and Euisik Yoon",

year = "2008",

doi = "10.1109/MEMSYS.2008.4443581",

language = "English",

isbn = "9781424417933",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "14--17",

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note = "21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson ; Conference date: 13-01-2008 Through 17-01-2008",

}

Kim, YJ, Chung, J, Lee, HK & Yoon, E 2008, Microfluidic array chip for single-cell isolation using two-way pneumatic actuation. in MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems., 4443581, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 14-17, 21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson, Tucson, AZ, United States, 08/1/13. https://doi.org/10.1109/MEMSYS.2008.4443581

Microfluidic array chip for single-cell isolation using two-way pneumatic actuation. / Kim, Young Ji; Chung, Jaehoon; Lee, Hyung Kew et al.

MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems. 2008. p. 14-17 4443581 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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N2 - In this work, we have implemented a new microfluidic chip which can capture and isolate single-cells in a microchamber array for various single-cell assays. Proposed microfluidic chip consists of two PDMS (Polydimethylsiloxane) layers: a microfluidic channel layer for injecting cells and reagents and an air channel for pneumatic actuation. Each microfluidic chip has 27 microchambers for single-cell culture and analysis. In order to verify our single-cell capturing scheme, we tested the fabricated chip with 15?m-diameter polystyrene microbeads. We successfully trapped the microbeads in each the capture site with a capture rate higher than 80% and loaded them into the corresponding chambers. After loading, the chambers were isolated from one another by pneumatic actuation. The isolation was confirmed by flowing blue ink into the main flow channel.

AB - In this work, we have implemented a new microfluidic chip which can capture and isolate single-cells in a microchamber array for various single-cell assays. Proposed microfluidic chip consists of two PDMS (Polydimethylsiloxane) layers: a microfluidic channel layer for injecting cells and reagents and an air channel for pneumatic actuation. Each microfluidic chip has 27 microchambers for single-cell culture and analysis. In order to verify our single-cell capturing scheme, we tested the fabricated chip with 15?m-diameter polystyrene microbeads. We successfully trapped the microbeads in each the capture site with a capture rate higher than 80% and loaded them into the corresponding chambers. After loading, the chambers were isolated from one another by pneumatic actuation. The isolation was confirmed by flowing blue ink into the main flow channel.

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Microfluidic array chip for single-cell isolation using two-way pneumatic actuation

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