Microfluidic assay-based optical measurement techniques for cell analysis: A review of recent progress

Jong ryul Choi, Hyerin Song, Jong Hwan Sung, Donghyun Kim, Kyujung Kim

Research output: Contribution to journalReview articlepeer-review

46 Citations (Scopus)

Abstract

Since the early 2000s, microfluidic cell culture systems have attracted significant attention as a promising alternative to conventional cell culture methods and the importance of designing an efficient detection system to analyze cell behavior on a chip in real time is raised. For this reason, various measurement techniques for microfluidic devices have been developed with the development of microfluidic assays for high-throughput screening and mimicking of in vivo conditions. In this review, we discuss optical measurement techniques for microfluidic assays. First of all, the recent development of fluorescence- and absorbance-based optical measurement systems is described. Next, advanced optical detection systems are introduced with respect to three emphases: 1) optimization for long-term, real-time, and in situ measurements; 2) performance improvements; and 3) multimodal analysis conjugations. Moreover, we explore presents future prospects for the establishment of optical detection systems following the development of complex, multi-dimensional microfluidic cell culture assays to mimic in vivo tissue, organ, and human systems.

Original languageEnglish
Pages (from-to)227-236
Number of pages10
JournalBiosensors and Bioelectronics
Volume77
DOIs
Publication statusPublished - 2016 Mar 15

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning ( 2014R1A1A1002810 , 2014R1A1A1004510 ). This work was also supported by Hongik University Research Fund and Small and Medium Business Administration (No. S2177822 ).

Publisher Copyright:
© 2015 Elsevier B.V..

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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