Abstract
We report cell-cell interaction studies utilizing a microfluidic chip capable of capturing cell pairs of different types into culture microwells and isolating the captured cells for a given amount of time using pneumatic valves. We have quantified the capture efficiency and confirmed cell viability and interaction in the microwells. We successfully distinguished interaction modes in the fabricated devices: secretion-based and contact-based. C2C12 cells, when co-cultured with PC3, showed increased proliferation due to PC3 secretions. Also, it was indentified that C2C12 proliferation is increased through contact with endothelial cells by comparing proliferation in both secretion only and contact only systems, respectively.
| Original language | English |
|---|---|
| Title of host publication | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
| Pages | 277-279 |
| Number of pages | 3 |
| Publication status | Published - 2010 Dec 1 |
| Event | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands Duration: 2010 Oct 3 → 2010 Oct 7 |
Publication series
| Name | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
|---|---|
| Volume | 1 |
Other
| Other | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
|---|---|
| Country | Netherlands |
| City | Groningen |
| Period | 10/10/3 → 10/10/7 |
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All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
Cite this
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Microfluidic assay to compare secretion vs contact based cell-cell interactions using dynamic isolation control. / Ingram, Patrick; Kim, Young Ji; Bersano-Begey, Tom; Lou, Xia; Asakura, Atsushi; Yoon, Euisik.
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 277-279 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 1).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Microfluidic assay to compare secretion vs contact based cell-cell interactions using dynamic isolation control
AU - Ingram, Patrick
AU - Kim, Young Ji
AU - Bersano-Begey, Tom
AU - Lou, Xia
AU - Asakura, Atsushi
AU - Yoon, Euisik
PY - 2010/12/1
Y1 - 2010/12/1
N2 - We report cell-cell interaction studies utilizing a microfluidic chip capable of capturing cell pairs of different types into culture microwells and isolating the captured cells for a given amount of time using pneumatic valves. We have quantified the capture efficiency and confirmed cell viability and interaction in the microwells. We successfully distinguished interaction modes in the fabricated devices: secretion-based and contact-based. C2C12 cells, when co-cultured with PC3, showed increased proliferation due to PC3 secretions. Also, it was indentified that C2C12 proliferation is increased through contact with endothelial cells by comparing proliferation in both secretion only and contact only systems, respectively.
AB - We report cell-cell interaction studies utilizing a microfluidic chip capable of capturing cell pairs of different types into culture microwells and isolating the captured cells for a given amount of time using pneumatic valves. We have quantified the capture efficiency and confirmed cell viability and interaction in the microwells. We successfully distinguished interaction modes in the fabricated devices: secretion-based and contact-based. C2C12 cells, when co-cultured with PC3, showed increased proliferation due to PC3 secretions. Also, it was indentified that C2C12 proliferation is increased through contact with endothelial cells by comparing proliferation in both secretion only and contact only systems, respectively.
UR - http://www.scopus.com/inward/record.url?scp=84860476850&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860476850&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84860476850
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 277
EP - 279
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
ER -