Microfluidic assay to compare secretion vs contact based cell-cell interactions using dynamic isolation control

Patrick Ingram, Young Ji Kim, Tom Bersano-Begey, Xia Lou, Atsushi Asakura, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

We report cell-cell interaction studies utilizing a microfluidic chip capable of capturing cell pairs of different types into culture microwells and isolating the captured cells for a given amount of time using pneumatic valves. We have quantified the capture efficiency and confirmed cell viability and interaction in the microwells. We successfully distinguished interaction modes in the fabricated devices: secretion-based and contact-based. C2C12 cells, when co-cultured with PC3, showed increased proliferation due to PC3 secretions. Also, it was indentified that C2C12 proliferation is increased through contact with endothelial cells by comparing proliferation in both secretion only and contact only systems, respectively.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages277-279
Number of pages3
Publication statusPublished - 2010 Dec 1
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume1

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/10/310/10/7

Fingerprint

Endothelial cells
Microfluidics
Pneumatics
Contacts (fluid mechanics)
Assays
Cells

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Ingram, P., Kim, Y. J., Bersano-Begey, T., Lou, X., Asakura, A., & Yoon, E. (2010). Microfluidic assay to compare secretion vs contact based cell-cell interactions using dynamic isolation control. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 277-279). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 1).
Ingram, Patrick ; Kim, Young Ji ; Bersano-Begey, Tom ; Lou, Xia ; Asakura, Atsushi ; Yoon, Euisik. / Microfluidic assay to compare secretion vs contact based cell-cell interactions using dynamic isolation control. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. pp. 277-279 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).
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abstract = "We report cell-cell interaction studies utilizing a microfluidic chip capable of capturing cell pairs of different types into culture microwells and isolating the captured cells for a given amount of time using pneumatic valves. We have quantified the capture efficiency and confirmed cell viability and interaction in the microwells. We successfully distinguished interaction modes in the fabricated devices: secretion-based and contact-based. C2C12 cells, when co-cultured with PC3, showed increased proliferation due to PC3 secretions. Also, it was indentified that C2C12 proliferation is increased through contact with endothelial cells by comparing proliferation in both secretion only and contact only systems, respectively.",
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Ingram, P, Kim, YJ, Bersano-Begey, T, Lou, X, Asakura, A & Yoon, E 2010, Microfluidic assay to compare secretion vs contact based cell-cell interactions using dynamic isolation control. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, vol. 1, pp. 277-279, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10/10/3.

Microfluidic assay to compare secretion vs contact based cell-cell interactions using dynamic isolation control. / Ingram, Patrick; Kim, Young Ji; Bersano-Begey, Tom; Lou, Xia; Asakura, Atsushi; Yoon, Euisik.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 277-279 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Ingram P, Kim YJ, Bersano-Begey T, Lou X, Asakura A, Yoon E. Microfluidic assay to compare secretion vs contact based cell-cell interactions using dynamic isolation control. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 277-279. (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).