Microfluidic assay to study transendothelial migration of human leukocyte

Sewoon Han, Ji Jing Yan, Yoojin Shin, Roger D. Kamm, Young-Joon Kim, Seok Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Most of the study on transendothelial migration has been conducted with 2D in vitro assays, which are not appropriate for high-resolution imaging of cell-cell interaction, quantification of the cell migration ability in time-dependency, and investigation of 3D cell migration. For these reasons, the complex transendothelial migration cascades have hardly ever discovered. To overcome these drawbacks of several established in vitro essays, here, we introduce the hydrogel scaffold embedded microfluidic platform. In this study, we have demonstrated the transendothelial migration of human leukocytes interacting with hMVECs monolayer with the precisely regulated chemotactic gradient of inflammatory mediators.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages822-824
Number of pages3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Fingerprint

Microfluidics
Hydrogels
Scaffolds
Monolayers
Assays
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Han, S., Yan, J. J., Shin, Y., Kamm, R. D., Kim, Y-J., & Chung, S. (2011). Microfluidic assay to study transendothelial migration of human leukocyte. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 822-824). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).
Han, Sewoon ; Yan, Ji Jing ; Shin, Yoojin ; Kamm, Roger D. ; Kim, Young-Joon ; Chung, Seok. / Microfluidic assay to study transendothelial migration of human leukocyte. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 822-824 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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abstract = "Most of the study on transendothelial migration has been conducted with 2D in vitro assays, which are not appropriate for high-resolution imaging of cell-cell interaction, quantification of the cell migration ability in time-dependency, and investigation of 3D cell migration. For these reasons, the complex transendothelial migration cascades have hardly ever discovered. To overcome these drawbacks of several established in vitro essays, here, we introduce the hydrogel scaffold embedded microfluidic platform. In this study, we have demonstrated the transendothelial migration of human leukocytes interacting with hMVECs monolayer with the precisely regulated chemotactic gradient of inflammatory mediators.",
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Han, S, Yan, JJ, Shin, Y, Kamm, RD, Kim, Y-J & Chung, S 2011, Microfluidic assay to study transendothelial migration of human leukocyte. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 2, pp. 822-824, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11/10/2.

Microfluidic assay to study transendothelial migration of human leukocyte. / Han, Sewoon; Yan, Ji Jing; Shin, Yoojin; Kamm, Roger D.; Kim, Young-Joon; Chung, Seok.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 822-824 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Han S, Yan JJ, Shin Y, Kamm RD, Kim Y-J, Chung S. Microfluidic assay to study transendothelial migration of human leukocyte. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 822-824. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).