TY - GEN
T1 - Microfluidic cell stimulator using bead impact
AU - Kim, Young Hun
AU - Kim, Tae Jin
AU - Kim, Hyung Joon
AU - Park, Min Suk
AU - Jung, Hyo I.
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009
Y1 - 2009
N2 - Recently many researchers are focused on cell stimulation regarding observation of cells to specific stimulation factors. We introduce new mechanical stimulation method using micro beads without any chemical reagents. CPAE (calf pulmonary artery endothelial cell) were cultured in PDMS (polydimethylsiloxane) microfluidic device. After starvation process, sterilized 10|im glass beads were rolled by only gravitational force for 10 minute. To find optimal stimulation time, 16 devices were made by PDMS and each device was slanted every hour. Results show that cell exposed under micro bead stimulation perform at a higher growth rate than normal conditions and 1 hour stimulation time represents more effective than other stimulation times. This new cell stimulation method will be able to help make artificial organ such as blood vessels in the future.
AB - Recently many researchers are focused on cell stimulation regarding observation of cells to specific stimulation factors. We introduce new mechanical stimulation method using micro beads without any chemical reagents. CPAE (calf pulmonary artery endothelial cell) were cultured in PDMS (polydimethylsiloxane) microfluidic device. After starvation process, sterilized 10|im glass beads were rolled by only gravitational force for 10 minute. To find optimal stimulation time, 16 devices were made by PDMS and each device was slanted every hour. Results show that cell exposed under micro bead stimulation perform at a higher growth rate than normal conditions and 1 hour stimulation time represents more effective than other stimulation times. This new cell stimulation method will be able to help make artificial organ such as blood vessels in the future.
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M3 - Conference contribution
AN - SCOPUS:67650558677
SN - 9789898111647
T3 - BIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices
SP - 426
EP - 429
BT - BIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices
T2 - 2nd International Conference on Biomedical Electronics and Devices, BIODEVICES 2009
Y2 - 14 January 2009 through 17 January 2009
ER -