Microfluidic chips designed for measuring biomolecules through a microbead-based quantum dot fluorescence assay.

Kwang Seok Yun, Dohoon Lee, Hak Sung Kim, Euisik Yoon

Research output: Contribution to journalArticlepeer-review

Abstract

This chapter introduces the demonstration of specific antibody detection by using a microbead-based assay with quantum dot (QD) fluorescence on a polydimethylsiloxane (PDMS) microfluidic chip. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies occurs on the surface. The microfluidic chip is fabricated on a glass substrate using a transparent silicone elastomer, PDMS, for easy access of monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assays has been performed in the fabricated chip, including the capturing of microbeads, antibody injection into a microchamber, quantum dot injection, and fluorescence detection. Various concentrations of human IgG antibodies have been introduced to bind to a single microbead captured and isolated inside a designated microchamber in a small volume of 75 pL. Fluorescence detection is monitored using a CCD camera after the second binding with the QDs conjugated with anti-human IgG. In this experiment, a human IgG antibody concentration below 0.1 microg/mL has been successfully detected.

Original languageEnglish
Pages (from-to)53-67
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume544
DOIs
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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