Microfluidic chips designed for measuring biomolecules through a microbead-based quantum dot fluorescence assay.

Kwang Seok Yun, Dohoon Lee, Hak Sung Kim, Euisik Yoon

Research output: Contribution to journalArticle

Abstract

This chapter introduces the demonstration of specific antibody detection by using a microbead-based assay with quantum dot (QD) fluorescence on a polydimethylsiloxane (PDMS) microfluidic chip. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies occurs on the surface. The microfluidic chip is fabricated on a glass substrate using a transparent silicone elastomer, PDMS, for easy access of monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assays has been performed in the fabricated chip, including the capturing of microbeads, antibody injection into a microchamber, quantum dot injection, and fluorescence detection. Various concentrations of human IgG antibodies have been introduced to bind to a single microbead captured and isolated inside a designated microchamber in a small volume of 75 pL. Fluorescence detection is monitored using a CCD camera after the second binding with the QDs conjugated with anti-human IgG. In this experiment, a human IgG antibody concentration below 0.1 microg/mL has been successfully detected.

Original languageEnglish
Pages (from-to)53-67
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume544
DOIs
Publication statusPublished - 2009

Fingerprint

Quantum Dots
Microfluidics
Microspheres
Fluorescence
Antibodies
Immunoglobulin G
Silicone Elastomers
Injections
Glass

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

@article{d54f7dcfebef435a8ad8303606d1246d,
title = "Microfluidic chips designed for measuring biomolecules through a microbead-based quantum dot fluorescence assay.",
abstract = "This chapter introduces the demonstration of specific antibody detection by using a microbead-based assay with quantum dot (QD) fluorescence on a polydimethylsiloxane (PDMS) microfluidic chip. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies occurs on the surface. The microfluidic chip is fabricated on a glass substrate using a transparent silicone elastomer, PDMS, for easy access of monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assays has been performed in the fabricated chip, including the capturing of microbeads, antibody injection into a microchamber, quantum dot injection, and fluorescence detection. Various concentrations of human IgG antibodies have been introduced to bind to a single microbead captured and isolated inside a designated microchamber in a small volume of 75 pL. Fluorescence detection is monitored using a CCD camera after the second binding with the QDs conjugated with anti-human IgG. In this experiment, a human IgG antibody concentration below 0.1 microg/mL has been successfully detected.",
author = "Yun, {Kwang Seok} and Dohoon Lee and Kim, {Hak Sung} and Euisik Yoon",
year = "2009",
doi = "10.1007/978-1-59745-483-4_5",
language = "English",
volume = "544",
pages = "53--67",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

Microfluidic chips designed for measuring biomolecules through a microbead-based quantum dot fluorescence assay. / Yun, Kwang Seok; Lee, Dohoon; Kim, Hak Sung; Yoon, Euisik.

In: Methods in molecular biology (Clifton, N.J.), Vol. 544, 2009, p. 53-67.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Microfluidic chips designed for measuring biomolecules through a microbead-based quantum dot fluorescence assay.

AU - Yun, Kwang Seok

AU - Lee, Dohoon

AU - Kim, Hak Sung

AU - Yoon, Euisik

PY - 2009

Y1 - 2009

N2 - This chapter introduces the demonstration of specific antibody detection by using a microbead-based assay with quantum dot (QD) fluorescence on a polydimethylsiloxane (PDMS) microfluidic chip. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies occurs on the surface. The microfluidic chip is fabricated on a glass substrate using a transparent silicone elastomer, PDMS, for easy access of monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assays has been performed in the fabricated chip, including the capturing of microbeads, antibody injection into a microchamber, quantum dot injection, and fluorescence detection. Various concentrations of human IgG antibodies have been introduced to bind to a single microbead captured and isolated inside a designated microchamber in a small volume of 75 pL. Fluorescence detection is monitored using a CCD camera after the second binding with the QDs conjugated with anti-human IgG. In this experiment, a human IgG antibody concentration below 0.1 microg/mL has been successfully detected.

AB - This chapter introduces the demonstration of specific antibody detection by using a microbead-based assay with quantum dot (QD) fluorescence on a polydimethylsiloxane (PDMS) microfluidic chip. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies occurs on the surface. The microfluidic chip is fabricated on a glass substrate using a transparent silicone elastomer, PDMS, for easy access of monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assays has been performed in the fabricated chip, including the capturing of microbeads, antibody injection into a microchamber, quantum dot injection, and fluorescence detection. Various concentrations of human IgG antibodies have been introduced to bind to a single microbead captured and isolated inside a designated microchamber in a small volume of 75 pL. Fluorescence detection is monitored using a CCD camera after the second binding with the QDs conjugated with anti-human IgG. In this experiment, a human IgG antibody concentration below 0.1 microg/mL has been successfully detected.

UR - http://www.scopus.com/inward/record.url?scp=70350767313&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350767313&partnerID=8YFLogxK

U2 - 10.1007/978-1-59745-483-4_5

DO - 10.1007/978-1-59745-483-4_5

M3 - Article

C2 - 19488693

AN - SCOPUS:70350767313

VL - 544

SP - 53

EP - 67

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -