Metastasis attributed to approximately 90% of cancer-related deaths; hence, the detection of metastatic tumor–derived components in the blood assists in determining cancer recurrence and patient survival. Microfluidic–based sensors facilitate analysis of small fluid volumes and represent an accurate, rapid, and user-friendly method of field diagnoses. In this study, we have developed a microfluidic chip-based exosomal mRNA sensor (exoNA-sensing chip) for the one-step detection of exosomal ERBB2 in the blood by integrating a microfluidic chip and 3D-nanostructured hydrogels. The exoNA-sensing chip is a vacuum-driven power-free microfluidic chip that can accurately control the flow of trace fluids (<100 μL). The sensing part of the exoNA-sensing chip includes 3D-nanostructured hydrogels capable of detecting ERBB2 and a reference gene by amplifying a fluorescent signal via an enzyme-free catalytic hairpin assembly reaction at room temperature. This hydrogel offers a detection limit of 58.3 fM with good selectivity for target sequences. The performance of the exoNA-sensing chip was evaluated by testing in vitro and in vivo samples and was proven to be effective for cancer diagnosis and liquid biopsies.
Bibliographical noteFunding Information:
This research was supported by the UST Young Scientist Research Program 2020 through the University of Science and Technology (No. 2020YS09 ), the Center for BioNano Health Guard funded by the MSIT as a Global Frontier Project ( HGUARD_2013M3A6B2078950 and HGUARD_2014M3A6B2060507 ), the Bio & Medical Technology Development Programs of the National Research Foundation (NRF) funded by MSIT (No. NRF-2018M3A9E2022819 , No. NRF-2018M3A9E2022821 , NRF-2019R1C1C1006867 , NRF-2020R1A2C1010453 and NRF-2021M3E5E3080379 ), Technology Development Program for Biological Hazards Management in Indoor Air through Korea Environment Industry & Technology Institute (KEITI) funded by Ministry of Environment (ME) of Korea ( 2021003370003 ), Industrial Technology Alchemist Program of the Ministry of Trade, Industry, and Energy (MOTIE) of Korea ( 20012435 ), and the KRIBB Research Initiative Program ( 1711134038 and 1711134081 )
All Science Journal Classification (ASJC) codes
- Biomedical Engineering