Microfluidic devices for the isolation of circulating rare cells

A focus on affinity-based, dielectrophoresis, and hydrophoresis

Kyung A. Hyun, Hyo il Jung

Research output: Contribution to journalReview article

40 Citations (Scopus)

Abstract

Circulating rare cells have attracted interest because they can be good indicators of various types of diseases. For example, enumeration of circulating tumor cells is used for cancer diagnosis and prognosis, while DNA analysis or enumeration of nucleated red blood cells is useful for prenatal diagnosis or hypoxic anemia, and that of circulating stem cells to diagnose cancer metastasis. Isolation of these cells and their downstream analyses can provide significant information such as the origin and characteristics of a disease. Novel approaches based on microfluidics have many advantages, including the continuous process and integration with other components for analysis. For these reasons, a variety of microfluidic devices have been developed to isolate and characterize rare cells. In this article, we review several microfluidic devices, with a focus on affinity-based isolation (e.g. antigen-antibody reaction) and label-free separation (DEP and hydrophoresis).

Original languageEnglish
Pages (from-to)1028-1041
Number of pages14
JournalElectrophoresis
Volume34
Issue number7
DOIs
Publication statusPublished - 2013 Apr 1

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Lab-On-A-Chip Devices
Electrophoresis
Microfluidics
Antigen-Antibody Reactions
Circulating Neoplastic Cells
Cell Separation
Prenatal Diagnosis
Cells
Antigen-antibody reactions
Anemia
Neoplasms
Stem Cells
Erythrocytes
Neoplasm Metastasis
Stem cells
Labels
Tumors
DNA
Blood

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

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abstract = "Circulating rare cells have attracted interest because they can be good indicators of various types of diseases. For example, enumeration of circulating tumor cells is used for cancer diagnosis and prognosis, while DNA analysis or enumeration of nucleated red blood cells is useful for prenatal diagnosis or hypoxic anemia, and that of circulating stem cells to diagnose cancer metastasis. Isolation of these cells and their downstream analyses can provide significant information such as the origin and characteristics of a disease. Novel approaches based on microfluidics have many advantages, including the continuous process and integration with other components for analysis. For these reasons, a variety of microfluidic devices have been developed to isolate and characterize rare cells. In this article, we review several microfluidic devices, with a focus on affinity-based isolation (e.g. antigen-antibody reaction) and label-free separation (DEP and hydrophoresis).",
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Microfluidic devices for the isolation of circulating rare cells : A focus on affinity-based, dielectrophoresis, and hydrophoresis. / Hyun, Kyung A.; Jung, Hyo il.

In: Electrophoresis, Vol. 34, No. 7, 01.04.2013, p. 1028-1041.

Research output: Contribution to journalReview article

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