Microfluidic high-throughput cell pairing and retrieval for studying breast cancer-MSC engulfment

Yu Chih Chen, Maria E. Gonzalez, Ayse Buke Hiziroglu, Xintao Zhao, Riley Brien, Yu Heng Cheng, Zhixiong Zhang, Celina G. Kleer, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Metastasis is the major cause of death in breast cancer patients. The interaction with mesenchymal stem/stromal cells promotes breast cancer metastasis. To investigate cellular engulfment in vitro, we have developed a high-throughput cell pairing and retrieval platform, which enables: (1) integration of 3,000 cell-pairing micro-chambers, allowing for contact of paired cancer cells and MSCs in each chamber, (2) automatic image collection and analysis to quantify engulfing rates, and (3) retrieval of target engulfing cells for downstream analysis.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages171-172
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2020 Jan 1
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 2017 Oct 222017 Oct 26

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period17/10/2217/10/26

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Chen, Y. C., Gonzalez, M. E., Hiziroglu, A. B., Zhao, X., Brien, R., Cheng, Y. H., Zhang, Z., Kleer, C. G., & Yoon, E. (2020). Microfluidic high-throughput cell pairing and retrieval for studying breast cancer-MSC engulfment. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 171-172). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.