Microfluidic high-throughput cell pairing and retrieval for studying breast cancer-MSC engulfment

Yu Chih Chen; Maria E. Gonzalez; Ayse Buke Hiziroglu; Xintao Zhao; Riley Brien; Yu Heng Cheng; Zhixiong Zhang; Celina G. Kleer; Euisik Yoon

Microfluidic high-throughput cell pairing and retrieval for studying breast cancer-MSC engulfment

Yu Chih Chen, Maria E. Gonzalez, Ayse Buke Hiziroglu, Xintao Zhao, Riley Brien, Yu Heng Cheng, Zhixiong Zhang, Celina G. Kleer, Euisik Yoon

Yonsei-IBS Institute

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Metastasis is the major cause of death in breast cancer patients. The interaction with mesenchymal stem/stromal cells promotes breast cancer metastasis. To investigate cellular engulfment in vitro, we have developed a high-throughput cell pairing and retrieval platform, which enables: (1) integration of 3,000 cell-pairing micro-chambers, allowing for contact of paired cancer cells and MSCs in each chamber, (2) automatic image collection and analysis to quantify engulfing rates, and (3) retrieval of target engulfing cells for downstream analysis.

Original language	English
Title of host publication	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Publisher	Chemical and Biological Microsystems Society
Pages	171-172
Number of pages	2
ISBN (Electronic)	9780692941836
Publication status	Published - 2020 Jan 1
Event	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States Duration: 2017 Oct 22 → 2017 Oct 26

Publication series

Name	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country	United States
City	Savannah
Period	17/10/22 → 17/10/26

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

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Chen, Y. C., Gonzalez, M. E., Hiziroglu, A. B., Zhao, X., Brien, R., Cheng, Y. H., Zhang, Z., Kleer, C. G., & Yoon, E. (2020). Microfluidic high-throughput cell pairing and retrieval for studying breast cancer-MSC engulfment. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 171-172). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.

Chen, Yu Chih ; Gonzalez, Maria E. ; Hiziroglu, Ayse Buke ; Zhao, Xintao ; Brien, Riley ; Cheng, Yu Heng ; Zhang, Zhixiong ; Kleer, Celina G. ; Yoon, Euisik. / Microfluidic high-throughput cell pairing and retrieval for studying breast cancer-MSC engulfment. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, 2020. pp. 171-172 (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

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title = "Microfluidic high-throughput cell pairing and retrieval for studying breast cancer-MSC engulfment",

abstract = "Metastasis is the major cause of death in breast cancer patients. The interaction with mesenchymal stem/stromal cells promotes breast cancer metastasis. To investigate cellular engulfment in vitro, we have developed a high-throughput cell pairing and retrieval platform, which enables: (1) integration of 3,000 cell-pairing micro-chambers, allowing for contact of paired cancer cells and MSCs in each chamber, (2) automatic image collection and analysis to quantify engulfing rates, and (3) retrieval of target engulfing cells for downstream analysis.",

author = "Chen, {Yu Chih} and Gonzalez, {Maria E.} and Hiziroglu, {Ayse Buke} and Xintao Zhao and Riley Brien and Cheng, {Yu Heng} and Zhixiong Zhang and Kleer, {Celina G.} and Euisik Yoon",

year = "2020",

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language = "English",

series = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",

publisher = "Chemical and Biological Microsystems Society",

pages = "171--172",

booktitle = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",

note = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 ; Conference date: 22-10-2017 Through 26-10-2017",

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Chen, YC, Gonzalez, ME, Hiziroglu, AB, Zhao, X, Brien, R, Cheng, YH, Zhang, Z, Kleer, CG & Yoon, E 2020, Microfluidic high-throughput cell pairing and retrieval for studying breast cancer-MSC engulfment. in 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, Chemical and Biological Microsystems Society, pp. 171-172, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, Savannah, United States, 17/10/22.

Microfluidic high-throughput cell pairing and retrieval for studying breast cancer-MSC engulfment. / Chen, Yu Chih; Gonzalez, Maria E.; Hiziroglu, Ayse Buke; Zhao, Xintao; Brien, Riley; Cheng, Yu Heng; Zhang, Zhixiong; Kleer, Celina G.; Yoon, Euisik.

21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, 2020. p. 171-172 (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Microfluidic high-throughput cell pairing and retrieval for studying breast cancer-MSC engulfment

AU - Chen, Yu Chih

AU - Gonzalez, Maria E.

AU - Hiziroglu, Ayse Buke

AU - Zhao, Xintao

AU - Brien, Riley

AU - Cheng, Yu Heng

AU - Zhang, Zhixiong

AU - Kleer, Celina G.

AU - Yoon, Euisik

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Metastasis is the major cause of death in breast cancer patients. The interaction with mesenchymal stem/stromal cells promotes breast cancer metastasis. To investigate cellular engulfment in vitro, we have developed a high-throughput cell pairing and retrieval platform, which enables: (1) integration of 3,000 cell-pairing micro-chambers, allowing for contact of paired cancer cells and MSCs in each chamber, (2) automatic image collection and analysis to quantify engulfing rates, and (3) retrieval of target engulfing cells for downstream analysis.

AB - Metastasis is the major cause of death in breast cancer patients. The interaction with mesenchymal stem/stromal cells promotes breast cancer metastasis. To investigate cellular engulfment in vitro, we have developed a high-throughput cell pairing and retrieval platform, which enables: (1) integration of 3,000 cell-pairing micro-chambers, allowing for contact of paired cancer cells and MSCs in each chamber, (2) automatic image collection and analysis to quantify engulfing rates, and (3) retrieval of target engulfing cells for downstream analysis.

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M3 - Conference contribution

AN - SCOPUS:85079695186

T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

SP - 171

EP - 172

BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

PB - Chemical and Biological Microsystems Society

T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Y2 - 22 October 2017 through 26 October 2017

ER -

Chen YC, Gonzalez ME, Hiziroglu AB, Zhao X, Brien R, Cheng YH et al. Microfluidic high-throughput cell pairing and retrieval for studying breast cancer-MSC engulfment. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society. 2020. p. 171-172. (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).