TY - GEN
T1 - Microfluidic high-throughput motility-based cell selection for enriching tumor initiating cells and discovering inhibition pathways of cancer migration
AU - Chen, Yu Chih
AU - Xiao, Annie
AU - Zhang, Zhixiong
AU - Luan, Yi
AU - Luker, Kathryn
AU - Luker, Gary
AU - Yoon, Euisik
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016
Y1 - 2016
N2 - Considerable evidence suggests that only a small subset of tumor-initiating cells (TICs) drive tumor formation and metastasis, and those cells have a strong correlation with cell motility. To enrich those TICs, we developed a high-throughput cell migration platform which enables: (1) integration of up to 5,000 migratory channels per device, (2) automatic image collection and analysis, and (3) reliable cell retrieval. Using this platform, we have retrieved highly motile cells for whole transcriptome analysis (mRNA Next Generation Sequencing) and in-vivo validation. Based on sequencing results, we identified the regulating pathways to inhibit cancer cell migration.
AB - Considerable evidence suggests that only a small subset of tumor-initiating cells (TICs) drive tumor formation and metastasis, and those cells have a strong correlation with cell motility. To enrich those TICs, we developed a high-throughput cell migration platform which enables: (1) integration of up to 5,000 migratory channels per device, (2) automatic image collection and analysis, and (3) reliable cell retrieval. Using this platform, we have retrieved highly motile cells for whole transcriptome analysis (mRNA Next Generation Sequencing) and in-vivo validation. Based on sequencing results, we identified the regulating pathways to inhibit cancer cell migration.
UR - http://www.scopus.com/inward/record.url?scp=85014156342&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014156342&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014156342
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 49
EP - 50
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -