Microfluidic high-throughput motility-based cell selection for enriching tumor initiating cells and discovering inhibition pathways of cancer migration

Yu Chih Chen, Annie Xiao, Zhixiong Zhang, Yi Luan, Kathryn Luker, Gary Luker, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Considerable evidence suggests that only a small subset of tumor-initiating cells (TICs) drive tumor formation and metastasis, and those cells have a strong correlation with cell motility. To enrich those TICs, we developed a high-throughput cell migration platform which enables: (1) integration of up to 5,000 migratory channels per device, (2) automatic image collection and analysis, and (3) reliable cell retrieval. Using this platform, we have retrieved highly motile cells for whole transcriptome analysis (mRNA Next Generation Sequencing) and in-vivo validation. Based on sequencing results, we identified the regulating pathways to inhibit cancer cell migration.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages49-50
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016 Jan 1
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

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All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Chen, Y. C., Xiao, A., Zhang, Z., Luan, Y., Luker, K., Luker, G., & Yoon, E. (2016). Microfluidic high-throughput motility-based cell selection for enriching tumor initiating cells and discovering inhibition pathways of cancer migration. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 49-50). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.