Microfluidic patterning of P-selectin for cell separation through rolling

Suman Bose, Seungpyo Hong, Robert Langer, Jeffrey M. Karp, Rohit Karnik

Research output: Contribution to conferencePaper

2 Citations (Scopus)

Abstract

We present a new method for label-free separation of cells by rolling on patterned P-selectin receptors. We patterned edges of P-selectin on a substrate using microfluidic channels and flowed a stream of cells and microspheres over the pattern at an angle to the patterns in a microfluidic device. The cells selectively interacted with P-selectin and were deflected from their path out of the flow stream by rolling on the patterned edges. This result provides proof-of-concept of separation and suggests that rolling on patterned receptors may be further developed into a simple, label- free system for separation of cells.

Original languageEnglish
Pages1205-1207
Number of pages3
Publication statusPublished - 2008 Jan 1
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

Fingerprint

P-Selectin
Microfluidics
Labels
Microspheres
Substrates
Selectins

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Bose, S., Hong, S., Langer, R., Karp, J. M., & Karnik, R. (2008). Microfluidic patterning of P-selectin for cell separation through rolling. 1205-1207. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.
Bose, Suman ; Hong, Seungpyo ; Langer, Robert ; Karp, Jeffrey M. ; Karnik, Rohit. / Microfluidic patterning of P-selectin for cell separation through rolling. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.3 p.
@conference{fb5ac55a21df44b2843ab9b9de117e02,
title = "Microfluidic patterning of P-selectin for cell separation through rolling",
abstract = "We present a new method for label-free separation of cells by rolling on patterned P-selectin receptors. We patterned edges of P-selectin on a substrate using microfluidic channels and flowed a stream of cells and microspheres over the pattern at an angle to the patterns in a microfluidic device. The cells selectively interacted with P-selectin and were deflected from their path out of the flow stream by rolling on the patterned edges. This result provides proof-of-concept of separation and suggests that rolling on patterned receptors may be further developed into a simple, label- free system for separation of cells.",
author = "Suman Bose and Seungpyo Hong and Robert Langer and Karp, {Jeffrey M.} and Rohit Karnik",
year = "2008",
month = "1",
day = "1",
language = "English",
pages = "1205--1207",
note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

}

Bose, S, Hong, S, Langer, R, Karp, JM & Karnik, R 2008, 'Microfluidic patterning of P-selectin for cell separation through rolling' Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 08/10/12 - 08/10/16, pp. 1205-1207.

Microfluidic patterning of P-selectin for cell separation through rolling. / Bose, Suman; Hong, Seungpyo; Langer, Robert; Karp, Jeffrey M.; Karnik, Rohit.

2008. 1205-1207 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conferencePaper

TY - CONF

T1 - Microfluidic patterning of P-selectin for cell separation through rolling

AU - Bose, Suman

AU - Hong, Seungpyo

AU - Langer, Robert

AU - Karp, Jeffrey M.

AU - Karnik, Rohit

PY - 2008/1/1

Y1 - 2008/1/1

N2 - We present a new method for label-free separation of cells by rolling on patterned P-selectin receptors. We patterned edges of P-selectin on a substrate using microfluidic channels and flowed a stream of cells and microspheres over the pattern at an angle to the patterns in a microfluidic device. The cells selectively interacted with P-selectin and were deflected from their path out of the flow stream by rolling on the patterned edges. This result provides proof-of-concept of separation and suggests that rolling on patterned receptors may be further developed into a simple, label- free system for separation of cells.

AB - We present a new method for label-free separation of cells by rolling on patterned P-selectin receptors. We patterned edges of P-selectin on a substrate using microfluidic channels and flowed a stream of cells and microspheres over the pattern at an angle to the patterns in a microfluidic device. The cells selectively interacted with P-selectin and were deflected from their path out of the flow stream by rolling on the patterned edges. This result provides proof-of-concept of separation and suggests that rolling on patterned receptors may be further developed into a simple, label- free system for separation of cells.

UR - http://www.scopus.com/inward/record.url?scp=84902436254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902436254&partnerID=8YFLogxK

M3 - Paper

SP - 1205

EP - 1207

ER -

Bose S, Hong S, Langer R, Karp JM, Karnik R. Microfluidic patterning of P-selectin for cell separation through rolling. 2008. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.