In this paper we have proposed and demonstrated a microfluidic device for a fast and parallel single-cell based assay. The proposed device is designed to passively capture single-cells or beads on multiple cell-positioning sites by a pre-defined fluidic stream and inject specific reagents or drugs onto each isolated single-cell. The device consists of surface-modified silicon channels capped with a grooved polydimethylsiloxane (PDMS) cover layer. A cell capture experiment has been performed using polystyrene beads as well as CHO DG44 living cells, and successful positioning of a single-cell on the cell-positioning sites was achieved. Also, we have demonstrated independent drug injection into a specific target cell.
Bibliographical noteFunding Information:
This research has been supported by the Intelligent Mi-crosystem Center, which carries out one of the 21st century’s Frontier R&D Projects sponsored by the Korea Ministry of Commerce, Industry and Energy. This work was supported by Brain Korea 21 Project, the School of Information Technology, KAIST in 2004. Also, this work has been partially supported by KOSEF through the MICROS center at KAIST.
All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- Molecular Biology