Micro/nanofluidic device for single-cell-based assay

Kwang Seok Yun, Euisik Yoon

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

In this paper we have proposed and demonstrated a microfluidic device for a fast and parallel single-cell based assay. The proposed device is designed to passively capture single-cells or beads on multiple cell-positioning sites by a pre-defined fluidic stream and inject specific reagents or drugs onto each isolated single-cell. The device consists of surface-modified silicon channels capped with a grooved polydimethylsiloxane (PDMS) cover layer. A cell capture experiment has been performed using polystyrene beads as well as CHO DG44 living cells, and successful positioning of a single-cell on the cell-positioning sites was achieved. Also, we have demonstrated independent drug injection into a specific target cell.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalBiomedical Microdevices
Volume7
Issue number1
DOIs
Publication statusPublished - 2005 Mar 1

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

Cite this