Microneedle-based minimally-invasive measurement of puncture resistance and fracture toughness of sclera

Seung Hyun Park, Kang Ju Lee, Ji Yong Lee, Jae Hyoung Yoon, Dong Hyun Jo, Jeong Hun Kim, Keon Wook Kang, WonHyoung Ryu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The sclera provides the structural support of the eye and protects the intraocular contents. Since it covers a large portion of the eye surface and has relatively high permeability for most drugs, the sclera has been used as a major pathway for drug administration. Recently, microneedle (MN) technology has shown the possibility of highly local and minimally-invasive drug delivery to the eye by MN insertion through the sclera or the suprachoroidal space. Although ocular MN needs to be inserted through the sclera, there has been no systematic study to understand the mechanical properties of the sclera, which are important to design ocular MNs. In this study, we investigated a MN-based method to measure the puncture resistance and fracture toughness of the sclera. To reflect the conditions of MN insertion into the sclera, force-displacement curves obtained from MN-insertion tests were used to estimate the puncture resistance and fracture toughness of sclera tissue. To understand the effect of the insertion conditions, dependency of the mechanical properties on insertion speeds, pre-strain of the sclera, and MN sizes were analyzed and discussed. Statement of Significance Measurement of mechanical property of soft biological tissue is challenging due to variations between tissue samples or lack of well-defined measurement techniques. Although non-invasive measurement techniques such as nano/micro indentation were employed to locally measure the elastic modulus of soft biological materials, mechanical properties such as puncture resistance or fracture toughness, which requires “invasive” measurement and is important for the application of “microneedles or hypodermic needles”, has not been well studied. In this work, we report minimally-invasive measurement of puncture resistance and fracture toughness of sclera using a double MN insertion method. Parametric studies showed that use of MN proved to be advantageous because of minimally-invasive insertion into tissue as well as higher sensitivity to sub-tissue architecture during the measurement.

Original languageEnglish
Pages (from-to)286-294
Number of pages9
JournalActa Biomaterialia
Volume44
DOIs
Publication statusPublished - 2016 Oct 15

Fingerprint

Sclera
Punctures
Fracture toughness
Tissue
Mechanical properties
Drug delivery
Indentation
Pharmaceutical Preparations
Needles
Biological materials
Elastic moduli
Elastic Modulus
Permeability
Technology

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Park, Seung Hyun ; Lee, Kang Ju ; Lee, Ji Yong ; Yoon, Jae Hyoung ; Jo, Dong Hyun ; Kim, Jeong Hun ; Kang, Keon Wook ; Ryu, WonHyoung. / Microneedle-based minimally-invasive measurement of puncture resistance and fracture toughness of sclera. In: Acta Biomaterialia. 2016 ; Vol. 44. pp. 286-294.
@article{083e5aa323e14610aa25f4cbb4578f8e,
title = "Microneedle-based minimally-invasive measurement of puncture resistance and fracture toughness of sclera",
abstract = "The sclera provides the structural support of the eye and protects the intraocular contents. Since it covers a large portion of the eye surface and has relatively high permeability for most drugs, the sclera has been used as a major pathway for drug administration. Recently, microneedle (MN) technology has shown the possibility of highly local and minimally-invasive drug delivery to the eye by MN insertion through the sclera or the suprachoroidal space. Although ocular MN needs to be inserted through the sclera, there has been no systematic study to understand the mechanical properties of the sclera, which are important to design ocular MNs. In this study, we investigated a MN-based method to measure the puncture resistance and fracture toughness of the sclera. To reflect the conditions of MN insertion into the sclera, force-displacement curves obtained from MN-insertion tests were used to estimate the puncture resistance and fracture toughness of sclera tissue. To understand the effect of the insertion conditions, dependency of the mechanical properties on insertion speeds, pre-strain of the sclera, and MN sizes were analyzed and discussed. Statement of Significance Measurement of mechanical property of soft biological tissue is challenging due to variations between tissue samples or lack of well-defined measurement techniques. Although non-invasive measurement techniques such as nano/micro indentation were employed to locally measure the elastic modulus of soft biological materials, mechanical properties such as puncture resistance or fracture toughness, which requires “invasive” measurement and is important for the application of “microneedles or hypodermic needles”, has not been well studied. In this work, we report minimally-invasive measurement of puncture resistance and fracture toughness of sclera using a double MN insertion method. Parametric studies showed that use of MN proved to be advantageous because of minimally-invasive insertion into tissue as well as higher sensitivity to sub-tissue architecture during the measurement.",
author = "Park, {Seung Hyun} and Lee, {Kang Ju} and Lee, {Ji Yong} and Yoon, {Jae Hyoung} and Jo, {Dong Hyun} and Kim, {Jeong Hun} and Kang, {Keon Wook} and WonHyoung Ryu",
year = "2016",
month = "10",
day = "15",
doi = "10.1016/j.actbio.2016.08.011",
language = "English",
volume = "44",
pages = "286--294",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",

}

Microneedle-based minimally-invasive measurement of puncture resistance and fracture toughness of sclera. / Park, Seung Hyun; Lee, Kang Ju; Lee, Ji Yong; Yoon, Jae Hyoung; Jo, Dong Hyun; Kim, Jeong Hun; Kang, Keon Wook; Ryu, WonHyoung.

In: Acta Biomaterialia, Vol. 44, 15.10.2016, p. 286-294.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Microneedle-based minimally-invasive measurement of puncture resistance and fracture toughness of sclera

AU - Park, Seung Hyun

AU - Lee, Kang Ju

AU - Lee, Ji Yong

AU - Yoon, Jae Hyoung

AU - Jo, Dong Hyun

AU - Kim, Jeong Hun

AU - Kang, Keon Wook

AU - Ryu, WonHyoung

PY - 2016/10/15

Y1 - 2016/10/15

N2 - The sclera provides the structural support of the eye and protects the intraocular contents. Since it covers a large portion of the eye surface and has relatively high permeability for most drugs, the sclera has been used as a major pathway for drug administration. Recently, microneedle (MN) technology has shown the possibility of highly local and minimally-invasive drug delivery to the eye by MN insertion through the sclera or the suprachoroidal space. Although ocular MN needs to be inserted through the sclera, there has been no systematic study to understand the mechanical properties of the sclera, which are important to design ocular MNs. In this study, we investigated a MN-based method to measure the puncture resistance and fracture toughness of the sclera. To reflect the conditions of MN insertion into the sclera, force-displacement curves obtained from MN-insertion tests were used to estimate the puncture resistance and fracture toughness of sclera tissue. To understand the effect of the insertion conditions, dependency of the mechanical properties on insertion speeds, pre-strain of the sclera, and MN sizes were analyzed and discussed. Statement of Significance Measurement of mechanical property of soft biological tissue is challenging due to variations between tissue samples or lack of well-defined measurement techniques. Although non-invasive measurement techniques such as nano/micro indentation were employed to locally measure the elastic modulus of soft biological materials, mechanical properties such as puncture resistance or fracture toughness, which requires “invasive” measurement and is important for the application of “microneedles or hypodermic needles”, has not been well studied. In this work, we report minimally-invasive measurement of puncture resistance and fracture toughness of sclera using a double MN insertion method. Parametric studies showed that use of MN proved to be advantageous because of minimally-invasive insertion into tissue as well as higher sensitivity to sub-tissue architecture during the measurement.

AB - The sclera provides the structural support of the eye and protects the intraocular contents. Since it covers a large portion of the eye surface and has relatively high permeability for most drugs, the sclera has been used as a major pathway for drug administration. Recently, microneedle (MN) technology has shown the possibility of highly local and minimally-invasive drug delivery to the eye by MN insertion through the sclera or the suprachoroidal space. Although ocular MN needs to be inserted through the sclera, there has been no systematic study to understand the mechanical properties of the sclera, which are important to design ocular MNs. In this study, we investigated a MN-based method to measure the puncture resistance and fracture toughness of the sclera. To reflect the conditions of MN insertion into the sclera, force-displacement curves obtained from MN-insertion tests were used to estimate the puncture resistance and fracture toughness of sclera tissue. To understand the effect of the insertion conditions, dependency of the mechanical properties on insertion speeds, pre-strain of the sclera, and MN sizes were analyzed and discussed. Statement of Significance Measurement of mechanical property of soft biological tissue is challenging due to variations between tissue samples or lack of well-defined measurement techniques. Although non-invasive measurement techniques such as nano/micro indentation were employed to locally measure the elastic modulus of soft biological materials, mechanical properties such as puncture resistance or fracture toughness, which requires “invasive” measurement and is important for the application of “microneedles or hypodermic needles”, has not been well studied. In this work, we report minimally-invasive measurement of puncture resistance and fracture toughness of sclera using a double MN insertion method. Parametric studies showed that use of MN proved to be advantageous because of minimally-invasive insertion into tissue as well as higher sensitivity to sub-tissue architecture during the measurement.

UR - http://www.scopus.com/inward/record.url?scp=84992159423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992159423&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2016.08.011

DO - 10.1016/j.actbio.2016.08.011

M3 - Article

C2 - 27521493

AN - SCOPUS:84992159423

VL - 44

SP - 286

EP - 294

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

ER -