Micropatterned assembly of silica nanoparticles for a protein microarray with enhanced detection sensitivity

Yeol Lee, Sangphil Park, Jin Won Park, Won-Gun Koh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We used an assembly of silica nanoparticles (SNPs) as a three-dimensional template for protein immobilization to prepare a protein microarray with enhanced protein loading capacity and detection sensitivity. SNPs were first modified with 3-aminopropyltriethoxysilane (APTES) for covalent immobilization of protein and micropatterned on poly(ethylene glycol)(PEG)-coated glass slides using elastomeric membranes with an array of holes. Proteins were selectively immobilized only on the SNP region, while the PEG regions served as an effective barrier to protein adsorption. Because of multi-layered SNPs that had curved surface, protein loading in the SNP micropattern was about six times greater than on a planar surface, as observed by fluorescence microscopy, which consequently improved the protein activity and reaction rate. GOX-catalyzed glucose oxidation and the molecular recognition mediated, specific binding between biotin and streptavidin were both successfully assayed using SNP microarrays, with better fluorescence signal and sensitivity than corresponding planar microarrays.

Original languageEnglish
Pages (from-to)457-464
Number of pages8
JournalBiomedical Microdevices
Volume12
Issue number3
DOIs
Publication statusPublished - 2010 Jun 1

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Protein Array Analysis
Microarrays
Silicon Dioxide
Nanoparticles
Silica
Proteins
Polyethylene glycols
Immobilization
Streptavidin
Ethylene Glycol
Biotin
Fluorescence Microscopy
Molecular recognition
Adsorption
Glass
Fluorescence microscopy
Membrane Proteins
Fluorescence
Reaction rates
Glucose

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

Cite this

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Micropatterned assembly of silica nanoparticles for a protein microarray with enhanced detection sensitivity. / Lee, Yeol; Park, Sangphil; Park, Jin Won; Koh, Won-Gun.

In: Biomedical Microdevices, Vol. 12, No. 3, 01.06.2010, p. 457-464.

Research output: Contribution to journalArticle

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