Microplate hybridization assay for detection of isoniazid resistance in Mycobacterium tuberculosis

Hye Eun Han, In Soo Lee, Joo Hwan Hwang, Hye Eun Bang, Yeun Kim, Sang Nae Cho, Tae Ue Kim, Hye Young Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Early and accurate detection of drug resistant Mycobacterium tuberculosis can improve both the treatment outcome and public health control of tuberculosis. A number of molecular-based techniques have been developed including ones using probe molecules that target drug resistance-related mutations. Although these techniques are highly specific and sensitive, mixed signals can be obtained when the drug resistant isolates are mixed with drug susceptible isolates. In order to overcome this problem, we developed a new drug susceptibility test (DST) for one of the most effective anti-tuberculosis drug, isoniazid. This technique employed a microplate hybridization assay that quantified signals from each probe molecule, and was evaluated using clinical isolates. The evaluation analysis clearly showed that the microplate hybridization assay was an accurate and rapid method that overcame the limitations of DST based on conventional molecular techniques.

Original languageEnglish
Pages (from-to)81-85
Number of pages5
JournalBMB reports
Volume42
Issue number2
DOIs
Publication statusPublished - 2009 Feb

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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