MicroRNA-Let-7a regulates the function of microglia in inflammation

Kyoung Joo Cho, Juhyun Song, Yumi Oh, Jongeun Lee

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Microglia have multiple functions in cerebrovascular and neurodegenerative diseases. Regulation of microglial function during inflammatory stress is important for treatment of central nervous system (CNS) diseases because microglia secrete various substances that affect neurons and glia. MicroRNA-Let-7a (miR-Let-7a) is a tumor suppressor miRNA that has been reported to target transcripts that encode proteins involved in apoptosis. In the present study, we examined the essential role of miR-Let-7a in inflammatory stress by over-expressing miR-Let-7a to investigate its role in determining the BV2 microglial phenotype, a cell line often used as a model of activated microglia. We found that inflammatory factors and Reactive Oxygen Species (ROS) production levels were altered according to miR-Let-7a expression level as measured by Western blot analysis, reverse transcription PCR, quantitative real time PCR, the measurement of nitrite (indicative of the nitric oxide (NO) pathway), and immunocytochemistry (ICC). Our results suggest that miR-Let-7a is involved in the function of microglia in the setting of inflammatory injury. In response to inflammation, miR-Let-7a participates in the reduction of nitrite production and the expression of inducible nitric oxide synthase (iNOS), interleukin (IL)-6 and is involved in increased expression of brain derived neurotrophic factor (BDNF), interleukin (IL)-10, and IL-4 in microglia. Thus, miRNA-Let-7a could act as a regulator of the function of microglia in inflammation.

Original languageEnglish
Pages (from-to)167-176
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume68
DOIs
Publication statusPublished - 2015 Sep 1

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Microglia
MicroRNAs
Inflammation
Nitrites
Cerebrovascular Disorders
Central Nervous System Diseases
Brain-Derived Neurotrophic Factor
Nitric Oxide Synthase Type II
Neuroglia
Interleukin-4
Neurodegenerative Diseases
Interleukin-10
Reverse Transcription
Real-Time Polymerase Chain Reaction
Interleukin-6
Reactive Oxygen Species
Nitric Oxide
Western Blotting
Immunohistochemistry
Apoptosis

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Cho, Kyoung Joo ; Song, Juhyun ; Oh, Yumi ; Lee, Jongeun. / MicroRNA-Let-7a regulates the function of microglia in inflammation. In: Molecular and Cellular Neuroscience. 2015 ; Vol. 68. pp. 167-176.
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MicroRNA-Let-7a regulates the function of microglia in inflammation. / Cho, Kyoung Joo; Song, Juhyun; Oh, Yumi; Lee, Jongeun.

In: Molecular and Cellular Neuroscience, Vol. 68, 01.09.2015, p. 167-176.

Research output: Contribution to journalArticle

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