MicroRNA profiling of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line

Eun Ji Nam, Maria Lee, Ga Won Yim, Jae Hoon Kim, Sunghoon Kim, Sang Wun Kim, Young Tae Kim

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background: Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. Cluster of differentiation (CD) 133+ and spheroid formation have been reported to be one of the main features of ovarian CSCs. Therefore, we determined the miRNA expression profile of a CD133 + spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line. Methods. Initially, we confirmed the enrichment of the OVCAR3 CD133 subpopulation by evaluating in vitro anchorage-independent growth. After obtaining a subpopulation of CD133+ OVCAR3 cells with>98% purity via cell sorting, miRNA microarray and real-time reverse transcription- polymerase chain reaction (RT-PCR) were performed to evaluate its miRNA profile. Results: We found 37 differentially expressed miRNAs in the CD133+ spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b, and miR-3, and 3 of which were significantly down-regulated, including miR-1202 and miR-1181. Conclusions: Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs.

Original languageEnglish
Article number18
JournalBMC Medical Genomics
Volume5
DOIs
Publication statusPublished - 2012 May 30

Fingerprint

MicroRNAs
Ovarian Neoplasms
Neoplastic Stem Cells
Cell Line
Stem Cells
Reverse Transcription
Carcinogenesis
Recurrence
Polymerase Chain Reaction
Growth
Neoplasms

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

@article{0069cb7bb849485d9dd6821731d59aa7,
title = "MicroRNA profiling of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line",
abstract = "Background: Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. Cluster of differentiation (CD) 133+ and spheroid formation have been reported to be one of the main features of ovarian CSCs. Therefore, we determined the miRNA expression profile of a CD133 + spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line. Methods. Initially, we confirmed the enrichment of the OVCAR3 CD133 subpopulation by evaluating in vitro anchorage-independent growth. After obtaining a subpopulation of CD133+ OVCAR3 cells with>98{\%} purity via cell sorting, miRNA microarray and real-time reverse transcription- polymerase chain reaction (RT-PCR) were performed to evaluate its miRNA profile. Results: We found 37 differentially expressed miRNAs in the CD133+ spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b, and miR-3, and 3 of which were significantly down-regulated, including miR-1202 and miR-1181. Conclusions: Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs.",
author = "Nam, {Eun Ji} and Maria Lee and Yim, {Ga Won} and Kim, {Jae Hoon} and Sunghoon Kim and Kim, {Sang Wun} and Kim, {Young Tae}",
year = "2012",
month = "5",
day = "30",
doi = "10.1186/1755-8794-5-18",
language = "English",
volume = "5",
journal = "BMC Medical Genomics",
issn = "1755-8794",
publisher = "BioMed Central",

}

MicroRNA profiling of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line. / Nam, Eun Ji; Lee, Maria; Yim, Ga Won; Kim, Jae Hoon; Kim, Sunghoon; Kim, Sang Wun; Kim, Young Tae.

In: BMC Medical Genomics, Vol. 5, 18, 30.05.2012.

Research output: Contribution to journalArticle

TY - JOUR

T1 - MicroRNA profiling of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line

AU - Nam, Eun Ji

AU - Lee, Maria

AU - Yim, Ga Won

AU - Kim, Jae Hoon

AU - Kim, Sunghoon

AU - Kim, Sang Wun

AU - Kim, Young Tae

PY - 2012/5/30

Y1 - 2012/5/30

N2 - Background: Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. Cluster of differentiation (CD) 133+ and spheroid formation have been reported to be one of the main features of ovarian CSCs. Therefore, we determined the miRNA expression profile of a CD133 + spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line. Methods. Initially, we confirmed the enrichment of the OVCAR3 CD133 subpopulation by evaluating in vitro anchorage-independent growth. After obtaining a subpopulation of CD133+ OVCAR3 cells with>98% purity via cell sorting, miRNA microarray and real-time reverse transcription- polymerase chain reaction (RT-PCR) were performed to evaluate its miRNA profile. Results: We found 37 differentially expressed miRNAs in the CD133+ spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b, and miR-3, and 3 of which were significantly down-regulated, including miR-1202 and miR-1181. Conclusions: Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs.

AB - Background: Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. Cluster of differentiation (CD) 133+ and spheroid formation have been reported to be one of the main features of ovarian CSCs. Therefore, we determined the miRNA expression profile of a CD133 + spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line. Methods. Initially, we confirmed the enrichment of the OVCAR3 CD133 subpopulation by evaluating in vitro anchorage-independent growth. After obtaining a subpopulation of CD133+ OVCAR3 cells with>98% purity via cell sorting, miRNA microarray and real-time reverse transcription- polymerase chain reaction (RT-PCR) were performed to evaluate its miRNA profile. Results: We found 37 differentially expressed miRNAs in the CD133+ spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b, and miR-3, and 3 of which were significantly down-regulated, including miR-1202 and miR-1181. Conclusions: Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs.

UR - http://www.scopus.com/inward/record.url?scp=84861466680&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861466680&partnerID=8YFLogxK

U2 - 10.1186/1755-8794-5-18

DO - 10.1186/1755-8794-5-18

M3 - Article

C2 - 22643117

AN - SCOPUS:84861466680

VL - 5

JO - BMC Medical Genomics

JF - BMC Medical Genomics

SN - 1755-8794

M1 - 18

ER -