Background: Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. Cluster of differentiation (CD) 133+ and spheroid formation have been reported to be one of the main features of ovarian CSCs. Therefore, we determined the miRNA expression profile of a CD133 + spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line. Methods. Initially, we confirmed the enrichment of the OVCAR3 CD133 subpopulation by evaluating in vitro anchorage-independent growth. After obtaining a subpopulation of CD133+ OVCAR3 cells with>98% purity via cell sorting, miRNA microarray and real-time reverse transcription- polymerase chain reaction (RT-PCR) were performed to evaluate its miRNA profile. Results: We found 37 differentially expressed miRNAs in the CD133+ spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b, and miR-3, and 3 of which were significantly down-regulated, including miR-1202 and miR-1181. Conclusions: Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs.
Bibliographical noteFunding Information:
This study was supported by grants from the Yonsei University Research Fund of 2010 (6-2010-0006), a Faculty Research Grant of Yonsei University College of Medicine 2009 (6-2009-0127), the National Research Foundation of Korea Grant funded by the Korean Government (2010-0004672 and 2011-0010800), and the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A084120) .
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