Microsphere-Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1-MMP

Minhee Ku, Hyun Joon Kim, Su Yee Yau, Nara Yoon, Nam Hee Kim, Jong In Yook, Jin Suck Suh, Dae Eun Kim, Jaemoon Yang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Biophysical properties are intimately connected to metastatic functions and aggressiveness in cancers. Especially, cellular stiffness is regarded as a biomarker for the understanding of metastatic potential and drug sensitivity. Here, protease-mediated changes of cortical stiffness are identified due to the deformation of cytoskeleton alignment at a cortex. For the past few decades, membrane type 1-matrix metalloproteinase (MT1-MMP) has been well known as a kernel protease enriched in podosomes during metastasis for extracellular matrix degradation. However, the biophysical significance of MT1-MMP expressing cancer cells is still unknown. Therefore, the nanomechanics of cancer cells is analyzed by a nanoindentation using a microsphere-attached cantilever of atomic force microscopy (AFM). In conclusion, the results suggest that MT1-MMP has contributed as a key regulator in cytoskeletal deformation related with cancer metastasis. Particularly, the AFM-based nanoindentation system for the monitoring of cortical nanomechanics will be crucial to understand molecular networks in cancers.

Original languageEnglish
Article number1803000
JournalSmall
Volume14
Issue number41
DOIs
Publication statusPublished - 2018 Oct 11

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Matrix Metalloproteinase 14
Nanoindentation
Nanomechanics
Microspheres
Stiffness
Membranes
Monitoring
Atomic force microscopy
Peptide Hydrolases
Cells
Atomic Force Microscopy
Neoplasms
Biomarkers
Neoplasm Metastasis
Degradation
Cytoskeleton
Extracellular Matrix
Pharmaceutical Preparations
Metalloproteases

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

Cite this

Ku, M., Kim, H. J., Yau, S. Y., Yoon, N., Kim, N. H., Yook, J. I., ... Yang, J. (2018). Microsphere-Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1-MMP. Small, 14(41), [1803000]. https://doi.org/10.1002/smll.201803000
Ku, Minhee ; Kim, Hyun Joon ; Yau, Su Yee ; Yoon, Nara ; Kim, Nam Hee ; Yook, Jong In ; Suh, Jin Suck ; Kim, Dae Eun ; Yang, Jaemoon. / Microsphere-Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1-MMP. In: Small. 2018 ; Vol. 14, No. 41.
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Microsphere-Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1-MMP. / Ku, Minhee; Kim, Hyun Joon; Yau, Su Yee; Yoon, Nara; Kim, Nam Hee; Yook, Jong In; Suh, Jin Suck; Kim, Dae Eun; Yang, Jaemoon.

In: Small, Vol. 14, No. 41, 1803000, 11.10.2018.

Research output: Contribution to journalArticle

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