Abstract
To evaluate the association between the concentrations of immune-related proteins in mid-trimester amniotic fluid (AF) and the subsequent risk of spontaneous preterm delivery in twins.Study Design:The study population consisted of consecutive women with a twin pregnancy who underwent clinically indicated genetic amniocentesis at 15 to 20 weeks, and had a subsequent spontaneous delivery in the early preterm period (<32 weeks (cases)) or at term (37 to 42 weeks (controls)). AF was analyzed for cytokines (interleukin (IL)-1ß, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13 and IL-15, interferon-γ, tumor necrosis factor-α), matrix metalloproteinases (MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 and MMP-12), and chemokines (complement factor-D/Adipsin, Serpin E1/PAI-1, Adiponectin/Acrp30, C-Reactive Protein, CCL2/MCP-1, Leptin, Resistin) using multiplex immunoassay kits. The association between AF protein levels and subsequent early preterm birth were examined.Result:A total of 96 sets of twins were enrolled, including 17 early preterm birth cases and 79 term controls. AF concentrations of IL-6, IL-8, MMP-3, MMP-8 and MMP-9, and CCL2/MCP-1 were significantly higher in cases than controls. Among these analytes, the combination of AF IL-8 and MMP-9 values had the highest predictive value for early preterm birth. The risk was 8% (10/132) for IL-8<1200 pg ml-1 and MMP-9<1000 pg ml-1, 30% (15/50) for IL-8>1200 pg ml-1 or MMP-9>1000 pg ml-1, and 90% (9/10) for IL-8>1200 pg ml-1 and MMP-9>1000 pg ml-1 (P<0.001).Conclusion:High concentrations of IL-8 and MMP-9 in mid-trimester AF in twins predicted well the risk of early preterm birth.
| Original language | English |
|---|---|
| Pages (from-to) | 542-546 |
| Number of pages | 5 |
| Journal | Journal of Perinatology |
| Volume | 35 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2015 Aug 30 |
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All Science Journal Classification (ASJC) codes
- Pediatrics, Perinatology, and Child Health
- Obstetrics and Gynaecology
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Mid-trimester amniotic fluid pro-inflammatory biomarkers predict the risk of spontaneous preterm delivery in twins : A retrospective cohort study. / Lee, S. M.; Park, J. S.; Norwitz, E. R.; Oh, S.; Kim, E. J.; Kim, S. M.; Lee, J.; Kim, B. J.; Park, C. W.; Jun, J. K.
In: Journal of Perinatology, Vol. 35, No. 8, 30.08.2015, p. 542-546.Research output: Contribution to journal › Article
TY - JOUR
T1 - Mid-trimester amniotic fluid pro-inflammatory biomarkers predict the risk of spontaneous preterm delivery in twins
T2 - A retrospective cohort study
AU - Lee, S. M.
AU - Park, J. S.
AU - Norwitz, E. R.
AU - Oh, S.
AU - Kim, E. J.
AU - Kim, S. M.
AU - Lee, J.
AU - Kim, B. J.
AU - Park, C. W.
AU - Jun, J. K.
PY - 2015/8/30
Y1 - 2015/8/30
N2 - To evaluate the association between the concentrations of immune-related proteins in mid-trimester amniotic fluid (AF) and the subsequent risk of spontaneous preterm delivery in twins.Study Design:The study population consisted of consecutive women with a twin pregnancy who underwent clinically indicated genetic amniocentesis at 15 to 20 weeks, and had a subsequent spontaneous delivery in the early preterm period (<32 weeks (cases)) or at term (37 to 42 weeks (controls)). AF was analyzed for cytokines (interleukin (IL)-1ß, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13 and IL-15, interferon-γ, tumor necrosis factor-α), matrix metalloproteinases (MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 and MMP-12), and chemokines (complement factor-D/Adipsin, Serpin E1/PAI-1, Adiponectin/Acrp30, C-Reactive Protein, CCL2/MCP-1, Leptin, Resistin) using multiplex immunoassay kits. The association between AF protein levels and subsequent early preterm birth were examined.Result:A total of 96 sets of twins were enrolled, including 17 early preterm birth cases and 79 term controls. AF concentrations of IL-6, IL-8, MMP-3, MMP-8 and MMP-9, and CCL2/MCP-1 were significantly higher in cases than controls. Among these analytes, the combination of AF IL-8 and MMP-9 values had the highest predictive value for early preterm birth. The risk was 8% (10/132) for IL-8<1200 pg ml-1 and MMP-9<1000 pg ml-1, 30% (15/50) for IL-8>1200 pg ml-1 or MMP-9>1000 pg ml-1, and 90% (9/10) for IL-8>1200 pg ml-1 and MMP-9>1000 pg ml-1 (P<0.001).Conclusion:High concentrations of IL-8 and MMP-9 in mid-trimester AF in twins predicted well the risk of early preterm birth.
AB - To evaluate the association between the concentrations of immune-related proteins in mid-trimester amniotic fluid (AF) and the subsequent risk of spontaneous preterm delivery in twins.Study Design:The study population consisted of consecutive women with a twin pregnancy who underwent clinically indicated genetic amniocentesis at 15 to 20 weeks, and had a subsequent spontaneous delivery in the early preterm period (<32 weeks (cases)) or at term (37 to 42 weeks (controls)). AF was analyzed for cytokines (interleukin (IL)-1ß, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13 and IL-15, interferon-γ, tumor necrosis factor-α), matrix metalloproteinases (MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 and MMP-12), and chemokines (complement factor-D/Adipsin, Serpin E1/PAI-1, Adiponectin/Acrp30, C-Reactive Protein, CCL2/MCP-1, Leptin, Resistin) using multiplex immunoassay kits. The association between AF protein levels and subsequent early preterm birth were examined.Result:A total of 96 sets of twins were enrolled, including 17 early preterm birth cases and 79 term controls. AF concentrations of IL-6, IL-8, MMP-3, MMP-8 and MMP-9, and CCL2/MCP-1 were significantly higher in cases than controls. Among these analytes, the combination of AF IL-8 and MMP-9 values had the highest predictive value for early preterm birth. The risk was 8% (10/132) for IL-8<1200 pg ml-1 and MMP-9<1000 pg ml-1, 30% (15/50) for IL-8>1200 pg ml-1 or MMP-9>1000 pg ml-1, and 90% (9/10) for IL-8>1200 pg ml-1 and MMP-9>1000 pg ml-1 (P<0.001).Conclusion:High concentrations of IL-8 and MMP-9 in mid-trimester AF in twins predicted well the risk of early preterm birth.
UR - http://www.scopus.com/inward/record.url?scp=84938285035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938285035&partnerID=8YFLogxK
U2 - 10.1038/jp.2015.29
DO - 10.1038/jp.2015.29
M3 - Article
C2 - 25856763
AN - SCOPUS:84938285035
VL - 35
SP - 542
EP - 546
JO - Journal of Perinatology
JF - Journal of Perinatology
SN - 0743-8346
IS - 8
ER -