Mind bomb-1 in dendritic cells is specifically required for notch-mediated t helper type 2 differentiation

Hyun Woo Jeong, Ji Hoon Kim, Joo Yeon Kim, Sang-Jun Ha, Young Yun Kong

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In dendritic cell (DC)-CD4 + T cell interaction, Notch signaling has been implicated in the CD4 + T cell activation, proliferation, and subset differentiation. However, there has been a lot of debate on the exact role of Notch signaling. Here, we observed that expression of Mind bomb-1 (Mib1), a critical regulator of Notch ligands for the activation of Notch signaling, increases gradually as precursor cells differentiate into DCs in mice. To clarify the role of Mib1 in DC-CD4 + T cell interactions, we generated Mib1-null bone marrow-derived DCs. These cells readily expressed Notch ligands but failed to initiate Notch activation in the adjacent cells. Nevertheless, Mib1-null DCs were able to prime the activation and proliferation of CD4 + T cells, suggesting that Notch activation in CD4 + T cells is not required for these processes. Intriguingly, stimulation of CD4 + T cells with Mib1-null DCs resulted in dramatically diminished Th2 cell populations, while preserving Th1 cell populations, both in vitro and in vivo. Our results demonstrate that Mib1 in DCs is critical for the activation of Notch signaling in CD4 + T cells, and Notch signaling reinforces Th2 differentiation, but is not required for the activation or proliferation of the CD4 + T cells.

Original languageEnglish
Article numbere36359
JournalPloS one
Volume7
Issue number4
DOIs
Publication statusPublished - 2012 Apr 27

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Bombs
T-cells
dendritic cells
Dendritic Cells
T-lymphocytes
T-Lymphocytes
Chemical activation
Cell Communication
cells
Ligands
Cells
Th2 Cells
Th1 Cells
T-Lymphocyte Subsets
Population
bone marrow
Bone Marrow
Cell Proliferation
Bone
mice

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Jeong, Hyun Woo ; Kim, Ji Hoon ; Kim, Joo Yeon ; Ha, Sang-Jun ; Kong, Young Yun. / Mind bomb-1 in dendritic cells is specifically required for notch-mediated t helper type 2 differentiation. In: PloS one. 2012 ; Vol. 7, No. 4.
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Mind bomb-1 in dendritic cells is specifically required for notch-mediated t helper type 2 differentiation. / Jeong, Hyun Woo; Kim, Ji Hoon; Kim, Joo Yeon; Ha, Sang-Jun; Kong, Young Yun.

In: PloS one, Vol. 7, No. 4, e36359, 27.04.2012.

Research output: Contribution to journalArticle

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