Mineralized polysaccharide transplantation modules supporting human MSC conversion into osteogenic cells and osteoid tissue in a non-union defect

Qing Ge, David William Green, Dong Joon Lee, Hyun Yi Kim, Zhengguo Piao, Jong Min Lee, Hansung Jung

Research output: Contribution to journalArticle

Abstract

Regenerative orthopedics needs significant devices to transplant human stem cells into damaged tissue and encourage automatic growth into replacements suitable for the human skeleton. Soft biomaterials have similarities in mechanical, structural and architectural properties to natural extracellular matrix (ECM), but often lack essential ECM molecules and signals. Here we engineer mineralized polysaccharide beads to transform MSCs into osteogenic cells and osteoid tissue for transplantation. Bone morphogenic proteins (BMP-2) and indispensable ECM proteins both directed differentiation inside alginate beads. Laminin and collagen IV basement membrane matrix proteins fixed and organized MSCs onto the alginate matrix, and BMP-2 drove differentiation, osteoid tissue self-assembly, and small-scale mineralization. Augmentation of alginate is necessary, and we showed that a few rationally selected small proteins from the basement membrane (BM) compartment of the ECM were sufficient to up-regulate cell expression of Runx-2 and osteocalcin for osteoid formation, resulting in Alizarin red-positive mineral nodules. More significantly, nested BMP-2 and BM beads added to a non-union skull defect, self-generated osteoid expressing osteopontin (OPN) and osteocalcin (OCN) in a chain along the defect, at only four weeks, establishing a framework for complete regeneration expected in 6 and 12 weeks. Alginate beads are beneficial surgical devices for transplanting therapeutic cells in programmed (by the ECM components and alginate-chitosan properties) reaction environments ideal for promoting bone tissue.

Original languageEnglish
Pages (from-to)1016-1023
Number of pages8
JournalMolecules and cells
Volume41
Issue number12
DOIs
Publication statusPublished - 2018 Jan 1

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Polysaccharides
Transplantation
Extracellular Matrix
Basement Membrane
Osteocalcin
Bone and Bones
Tissue Transplantation
Equipment and Supplies
Osteopontin
Extracellular Matrix Proteins
Chitosan
Biocompatible Materials
Laminin
Skull
Skeleton
Orthopedics
Minerals
Regeneration
Membrane Proteins
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Ge, Qing ; Green, David William ; Lee, Dong Joon ; Kim, Hyun Yi ; Piao, Zhengguo ; Lee, Jong Min ; Jung, Hansung. / Mineralized polysaccharide transplantation modules supporting human MSC conversion into osteogenic cells and osteoid tissue in a non-union defect. In: Molecules and cells. 2018 ; Vol. 41, No. 12. pp. 1016-1023.
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Mineralized polysaccharide transplantation modules supporting human MSC conversion into osteogenic cells and osteoid tissue in a non-union defect. / Ge, Qing; Green, David William; Lee, Dong Joon; Kim, Hyun Yi; Piao, Zhengguo; Lee, Jong Min; Jung, Hansung.

In: Molecules and cells, Vol. 41, No. 12, 01.01.2018, p. 1016-1023.

Research output: Contribution to journalArticle

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