Mineralocorticoid and glucocorticoid receptor expressions in astrocytes and microglia in the gerbil hippocampal CA1 region after ischemic insult

In Koo Hwang, Ki Yeon Yoo, Young Sam Nam, Jung Hoon Choi, In Se Lee, Young Guen Kwon, Tae Cheon Kang, Yong Sun Kim, Moo Ho Won

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

In the present study, we observed expression and changes of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in the gerbil hippocampal CA1 region, but not in the CA2/3 region, after 5 min of transient forebrain ischemia. In blood, corticosterone levels were increased biphasically at 30 min and 12 h after ischemia/reperfusion, and thereafter its levels were decreased. In the sham-operated group, MR and GR immunoreactivities were weakly detected in the CA1 region. By 3 days after ischemia, MR and GR were not significantly altered in the CA1 region: at 12 h after ischemia, GR was expressed in a few neurons in the CA1 region, whereas MR was not expressed in any neurons after ischemic insult. From 4 days after ischemia, MR and GR immunoreactivities were detected in astrocytes and microglia in the CA1 region, and at 7 days after ischemia, MR and GR immunoreactivities peaked in the hippocampal CA1 region. At this time, 55% of astrocytes and 30% of microglia showed MR immunoreactivity, and 20% of astrocytes and 40% of microglia showed GR immunoreactivity. Western blot analyses showed that the pattern of changes in MR and GR protein levels was similar to the immunohistochemical changes observed after transient forebrain ischemia. From 4 days after ischemia, MR and GR protein levels were increased time-dependently after ischemia. In conclusion, enhanced MR and GR expressions in astrocytes and microglia were detected in the hippocampal CA1 region 4-7 days after ischemia/reperfusion. At this time, GR immunoreactivity was abundant in microglia, whereas MR immunoreactivity was prominent in astrocytes. The specific distribution of corticosteroid receptors in the astrocytes and microglia may be associated with the differences of MR and GR functions against ischemic damage.

Original languageEnglish
Pages (from-to)319-327
Number of pages9
JournalNeuroscience Research
Volume54
Issue number4
DOIs
Publication statusPublished - 2006 Apr 1

Fingerprint

Hippocampal CA1 Region
Mineralocorticoid Receptors
Gerbillinae
Glucocorticoid Receptors
Microglia
Astrocytes
Ischemia
Prosencephalon
Reperfusion
Neurons
Steroid Receptors
Corticosterone
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Hwang, In Koo ; Yoo, Ki Yeon ; Nam, Young Sam ; Choi, Jung Hoon ; Lee, In Se ; Kwon, Young Guen ; Kang, Tae Cheon ; Kim, Yong Sun ; Won, Moo Ho. / Mineralocorticoid and glucocorticoid receptor expressions in astrocytes and microglia in the gerbil hippocampal CA1 region after ischemic insult. In: Neuroscience Research. 2006 ; Vol. 54, No. 4. pp. 319-327.
@article{1a39471f7490488388ce001c995ae280,
title = "Mineralocorticoid and glucocorticoid receptor expressions in astrocytes and microglia in the gerbil hippocampal CA1 region after ischemic insult",
abstract = "In the present study, we observed expression and changes of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in the gerbil hippocampal CA1 region, but not in the CA2/3 region, after 5 min of transient forebrain ischemia. In blood, corticosterone levels were increased biphasically at 30 min and 12 h after ischemia/reperfusion, and thereafter its levels were decreased. In the sham-operated group, MR and GR immunoreactivities were weakly detected in the CA1 region. By 3 days after ischemia, MR and GR were not significantly altered in the CA1 region: at 12 h after ischemia, GR was expressed in a few neurons in the CA1 region, whereas MR was not expressed in any neurons after ischemic insult. From 4 days after ischemia, MR and GR immunoreactivities were detected in astrocytes and microglia in the CA1 region, and at 7 days after ischemia, MR and GR immunoreactivities peaked in the hippocampal CA1 region. At this time, 55{\%} of astrocytes and 30{\%} of microglia showed MR immunoreactivity, and 20{\%} of astrocytes and 40{\%} of microglia showed GR immunoreactivity. Western blot analyses showed that the pattern of changes in MR and GR protein levels was similar to the immunohistochemical changes observed after transient forebrain ischemia. From 4 days after ischemia, MR and GR protein levels were increased time-dependently after ischemia. In conclusion, enhanced MR and GR expressions in astrocytes and microglia were detected in the hippocampal CA1 region 4-7 days after ischemia/reperfusion. At this time, GR immunoreactivity was abundant in microglia, whereas MR immunoreactivity was prominent in astrocytes. The specific distribution of corticosteroid receptors in the astrocytes and microglia may be associated with the differences of MR and GR functions against ischemic damage.",
author = "Hwang, {In Koo} and Yoo, {Ki Yeon} and Nam, {Young Sam} and Choi, {Jung Hoon} and Lee, {In Se} and Kwon, {Young Guen} and Kang, {Tae Cheon} and Kim, {Yong Sun} and Won, {Moo Ho}",
year = "2006",
month = "4",
day = "1",
doi = "10.1016/j.neures.2005.12.012",
language = "English",
volume = "54",
pages = "319--327",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",
number = "4",

}

Mineralocorticoid and glucocorticoid receptor expressions in astrocytes and microglia in the gerbil hippocampal CA1 region after ischemic insult. / Hwang, In Koo; Yoo, Ki Yeon; Nam, Young Sam; Choi, Jung Hoon; Lee, In Se; Kwon, Young Guen; Kang, Tae Cheon; Kim, Yong Sun; Won, Moo Ho.

In: Neuroscience Research, Vol. 54, No. 4, 01.04.2006, p. 319-327.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mineralocorticoid and glucocorticoid receptor expressions in astrocytes and microglia in the gerbil hippocampal CA1 region after ischemic insult

AU - Hwang, In Koo

AU - Yoo, Ki Yeon

AU - Nam, Young Sam

AU - Choi, Jung Hoon

AU - Lee, In Se

AU - Kwon, Young Guen

AU - Kang, Tae Cheon

AU - Kim, Yong Sun

AU - Won, Moo Ho

PY - 2006/4/1

Y1 - 2006/4/1

N2 - In the present study, we observed expression and changes of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in the gerbil hippocampal CA1 region, but not in the CA2/3 region, after 5 min of transient forebrain ischemia. In blood, corticosterone levels were increased biphasically at 30 min and 12 h after ischemia/reperfusion, and thereafter its levels were decreased. In the sham-operated group, MR and GR immunoreactivities were weakly detected in the CA1 region. By 3 days after ischemia, MR and GR were not significantly altered in the CA1 region: at 12 h after ischemia, GR was expressed in a few neurons in the CA1 region, whereas MR was not expressed in any neurons after ischemic insult. From 4 days after ischemia, MR and GR immunoreactivities were detected in astrocytes and microglia in the CA1 region, and at 7 days after ischemia, MR and GR immunoreactivities peaked in the hippocampal CA1 region. At this time, 55% of astrocytes and 30% of microglia showed MR immunoreactivity, and 20% of astrocytes and 40% of microglia showed GR immunoreactivity. Western blot analyses showed that the pattern of changes in MR and GR protein levels was similar to the immunohistochemical changes observed after transient forebrain ischemia. From 4 days after ischemia, MR and GR protein levels were increased time-dependently after ischemia. In conclusion, enhanced MR and GR expressions in astrocytes and microglia were detected in the hippocampal CA1 region 4-7 days after ischemia/reperfusion. At this time, GR immunoreactivity was abundant in microglia, whereas MR immunoreactivity was prominent in astrocytes. The specific distribution of corticosteroid receptors in the astrocytes and microglia may be associated with the differences of MR and GR functions against ischemic damage.

AB - In the present study, we observed expression and changes of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in the gerbil hippocampal CA1 region, but not in the CA2/3 region, after 5 min of transient forebrain ischemia. In blood, corticosterone levels were increased biphasically at 30 min and 12 h after ischemia/reperfusion, and thereafter its levels were decreased. In the sham-operated group, MR and GR immunoreactivities were weakly detected in the CA1 region. By 3 days after ischemia, MR and GR were not significantly altered in the CA1 region: at 12 h after ischemia, GR was expressed in a few neurons in the CA1 region, whereas MR was not expressed in any neurons after ischemic insult. From 4 days after ischemia, MR and GR immunoreactivities were detected in astrocytes and microglia in the CA1 region, and at 7 days after ischemia, MR and GR immunoreactivities peaked in the hippocampal CA1 region. At this time, 55% of astrocytes and 30% of microglia showed MR immunoreactivity, and 20% of astrocytes and 40% of microglia showed GR immunoreactivity. Western blot analyses showed that the pattern of changes in MR and GR protein levels was similar to the immunohistochemical changes observed after transient forebrain ischemia. From 4 days after ischemia, MR and GR protein levels were increased time-dependently after ischemia. In conclusion, enhanced MR and GR expressions in astrocytes and microglia were detected in the hippocampal CA1 region 4-7 days after ischemia/reperfusion. At this time, GR immunoreactivity was abundant in microglia, whereas MR immunoreactivity was prominent in astrocytes. The specific distribution of corticosteroid receptors in the astrocytes and microglia may be associated with the differences of MR and GR functions against ischemic damage.

UR - http://www.scopus.com/inward/record.url?scp=33644849813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644849813&partnerID=8YFLogxK

U2 - 10.1016/j.neures.2005.12.012

DO - 10.1016/j.neures.2005.12.012

M3 - Article

C2 - 16473422

AN - SCOPUS:33644849813

VL - 54

SP - 319

EP - 327

JO - Neuroscience Research

JF - Neuroscience Research

SN - 0168-0102

IS - 4

ER -