Miniaturization of frit inlet asymmetrical flow field-flow fractionation

Dukjin Kang, Myeong Hee Moon

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFIFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFIFFF system, flow rate ranges that can be manipulated were decreased to 20-30 μL/min, which reduces the injection amount of sample materials. The end effect contribution to plate height was evaluated by varying the inner diameter of the connection tubing between the injector and the channel inlet at various injection flow rates, and the results showed that the use of silica capillary tubing of the shortest possible distance is essential in reducing the initial band broadening prior to the sample injection to the microscale channel. The capability of the μFI-AFIFFF system was demonstrated with the separation of protein standards, polystyrene-sulfonates, and ssDNA strains and for the characterization of replication protein A-ssDNA binding complex regulated by redox status.

Original languageEnglish
Pages (from-to)3851-3855
Number of pages5
JournalAnalytical Chemistry
Volume76
Issue number13
DOIs
Publication statusPublished - 2004 Jul 1

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Inlet flow
Tubing
Fractionation
Flow fields
Replication Protein A
Flow rate
Silicon Dioxide
Proteins
polystyrene sulfonic acid
Oxidation-Reduction

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

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abstract = "A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFIFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFIFFF system, flow rate ranges that can be manipulated were decreased to 20-30 μL/min, which reduces the injection amount of sample materials. The end effect contribution to plate height was evaluated by varying the inner diameter of the connection tubing between the injector and the channel inlet at various injection flow rates, and the results showed that the use of silica capillary tubing of the shortest possible distance is essential in reducing the initial band broadening prior to the sample injection to the microscale channel. The capability of the μFI-AFIFFF system was demonstrated with the separation of protein standards, polystyrene-sulfonates, and ssDNA strains and for the characterization of replication protein A-ssDNA binding complex regulated by redox status.",
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Miniaturization of frit inlet asymmetrical flow field-flow fractionation. / Kang, Dukjin; Moon, Myeong Hee.

In: Analytical Chemistry, Vol. 76, No. 13, 01.07.2004, p. 3851-3855.

Research output: Contribution to journalArticle

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AU - Moon, Myeong Hee

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