Miniaturized asymmetrical flow field-flow fractionation: Application to biological vesicles

Sunok Oh, Dukjin Kang, Sung Min Ahn, Richard J. Simpson, Bong Hee Lee, Myeong Hee Moon

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Asymmetrical flow field-flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size. By reducing the channel dimension, proteins or particulate materials can be separated within a few minutes without a significant loss in resolution. The mAFlFFF channel was applied for the separation of exosomes harvested from immortalized human mesenchymal stem cell line. It shows a potential to fractionate exosome vesicles according to sizes which can be useful for proteomic studies in relation to immunotherapeutic applications.

Original languageEnglish
Pages (from-to)1082-1087
Number of pages6
JournalJournal of Separation Science
Volume30
Issue number7
DOIs
Publication statusPublished - 2007 May 1

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

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