The fluorescence quenching of CdSe/CdS core/shell quantum dots (QDs) with various shell thicknesses is investigated. Inducing aggregation of the core/shell QDs with the addition of NaCl shows that the fluorescence quenching efficiency decreases monotonically as the CdS shell thickness increases because of the reduced spectral overlap between the emission and absorption spectra. In addition, hybridization between the oligonucleotide-functionalized QDs and its complementary DNA with the fluorescent dye is used to study the fluorescence quenching caused by the Förster resonance energy transfer (FRET) and any uncontrolled aggregation. These experiments allow quantitative estimation of the effects of FRET and uncontrolled aggregation on the fluorescence quenching efficiency, which can be used to determine conditions to minimize undesirable fluorescence quenching due to uncontrolled aggregation in energy transfer-based biosensor applications.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation of Korea (NRF) grants (Nos. 2014R1A2A1A11051436 and 2012R1A2A2A01004416 ).
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry