MiR-200b regulates cell migration via Zeb family during mouse palate development

Jeong Oh Shin, Eizo Nakagawa, Eun Jung Kim, Kyoung Won Cho, Jong Min Lee, Sung Won Cho, Han Sung Jung

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Palate development requires coordinating proper cellular and molecular events in palatogenesis, including the epithelial-mesenchymal transition (EMT), apoptosis, cell proliferation, and cell migration. Zeb1 and Zeb2 regulate epithelial cadherin (E-cadherin) and EMT during organogenesis. While microRNA 200b (miR-200b) is known to be a negative regulator of Zeb1 and Zeb2 in cancer progression, its regulatory eVects on Zeb1 and Zeb2 in palatogenesis have not yet been clariWed. The aim of this study is to investigate the relationship between the regulators of palatal development, speciWcally, miR-200b and the Zeb family. Expression of both Zeb1 and Zeb2 was detected in the mesenchyme of the mouse palate, while miR-200b was expressed in the medial edge epithelium. After contact with the palatal shelves, miR-200b was expressed in the palatal epithelial lining and epithelial island around the fusion region but not in the palatal mesenchyme. The function of miR-200b was examined by overexpression via a lentiviral vector in the palatal shelves. Ectopic expression of miR-200b resulted in suppression of the Zeb family, upregulation of E-cadherin, and changes in cell migration and palatal fusion. These results suggest that miR-200b plays crucial roles in cell migration and palatal fusion by regulating Zeb1 and Zeb2 as a noncoding RNA during palate development.

Original languageEnglish
Pages (from-to)459-470
Number of pages12
JournalHistochemistry and cell biology
Volume137
Issue number4
DOIs
Publication statusPublished - 2012 Apr 1

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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