MISC-1/OGC links mitochondrial metabolism, apoptosis and insulin secretion

Marco Gallo, Donha Park, Dan S. Luciani, Katarzyna Kida, Ferdinando Palmieri, Oliver E. Blacque, James D. Johnson, Donald L. Riddle

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We identified MISC-1 (Mitochondrial Solute Carrier) as the C. elegans orthologue of mammalian OGC (2-oxoglutarate carrier). OGC was originally identified for its ability to transfer α-ketoglutarate across the inner mitochondrial membrane. However, we found that MISC-1 and OGC are not solely involved in metabolic control. Our data show that these orthologous proteins participate in phylogenetically conserved cellular processes, like control of mitochondrial morphology and induction of apoptosis. We show that MISC-1/OGC is required for proper mitochondrial fusion and fission events in both C. elegans and human cells. Transmission electron microscopy reveals that loss of MISC-1 results in a decreased number of mitochondrial cristae, which have a blebbed appearance. Furthermore, our pull-down experiments show that MISC-1 and OGC interact with the anti-apoptotic proteins CED-9 and Bcl-xL, respectively, and with the pro-apoptotic protein ANT. Knock-down of misc-1 in C. elegans and OGC in mouse cells induces apoptosis through the caspase cascade. Genetic analysis suggests that MISC-1 controls apoptosis through the physiological pathway mediated by the LIN-35/Rb-like protein. We provide genetic and molecular evidence that absence of MISC-1 increases insulin secretion and enhances germline stem cell proliferation in C. elegans. Our study suggests that the mitochondrial metabolic protein MISC-1/OGC integrates metabolic, apoptotic and insulin secretion functions. We propose a novel mechanism by which mitochondria integrate metabolic and cell survival signals. Our data suggest that MISC-1/OGC functions by sensing the metabolic status of mitochondria and directly activate the apoptotic program when required. Our results suggest that controlling MISC-1/OGC function allows regulation of mitochondrial morphology and cell survival decisions by the metabolic needs of the cell.

Original languageEnglish
Article numbere17827
JournalPloS one
Volume6
Issue number3
DOIs
Publication statusPublished - 2011 Mar 28

Fingerprint

insulin secretion
Metabolism
apoptosis
Insulin
Apoptosis
metabolism
cell viability
mitochondria
proteins
caspases
cells
solutes
transmission electron microscopy
genetic techniques and protocols
stem cells
germ cells
cell proliferation
Apoptosis Regulatory Proteins
Mitochondria
Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Gallo, M., Park, D., Luciani, D. S., Kida, K., Palmieri, F., Blacque, O. E., ... Riddle, D. L. (2011). MISC-1/OGC links mitochondrial metabolism, apoptosis and insulin secretion. PloS one, 6(3), [e17827]. https://doi.org/10.1371/journal.pone.0017827
Gallo, Marco ; Park, Donha ; Luciani, Dan S. ; Kida, Katarzyna ; Palmieri, Ferdinando ; Blacque, Oliver E. ; Johnson, James D. ; Riddle, Donald L. / MISC-1/OGC links mitochondrial metabolism, apoptosis and insulin secretion. In: PloS one. 2011 ; Vol. 6, No. 3.
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Gallo, M, Park, D, Luciani, DS, Kida, K, Palmieri, F, Blacque, OE, Johnson, JD & Riddle, DL 2011, 'MISC-1/OGC links mitochondrial metabolism, apoptosis and insulin secretion', PloS one, vol. 6, no. 3, e17827. https://doi.org/10.1371/journal.pone.0017827

MISC-1/OGC links mitochondrial metabolism, apoptosis and insulin secretion. / Gallo, Marco; Park, Donha; Luciani, Dan S.; Kida, Katarzyna; Palmieri, Ferdinando; Blacque, Oliver E.; Johnson, James D.; Riddle, Donald L.

In: PloS one, Vol. 6, No. 3, e17827, 28.03.2011.

Research output: Contribution to journalArticle

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AU - Gallo, Marco

AU - Park, Donha

AU - Luciani, Dan S.

AU - Kida, Katarzyna

AU - Palmieri, Ferdinando

AU - Blacque, Oliver E.

AU - Johnson, James D.

AU - Riddle, Donald L.

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AB - We identified MISC-1 (Mitochondrial Solute Carrier) as the C. elegans orthologue of mammalian OGC (2-oxoglutarate carrier). OGC was originally identified for its ability to transfer α-ketoglutarate across the inner mitochondrial membrane. However, we found that MISC-1 and OGC are not solely involved in metabolic control. Our data show that these orthologous proteins participate in phylogenetically conserved cellular processes, like control of mitochondrial morphology and induction of apoptosis. We show that MISC-1/OGC is required for proper mitochondrial fusion and fission events in both C. elegans and human cells. Transmission electron microscopy reveals that loss of MISC-1 results in a decreased number of mitochondrial cristae, which have a blebbed appearance. Furthermore, our pull-down experiments show that MISC-1 and OGC interact with the anti-apoptotic proteins CED-9 and Bcl-xL, respectively, and with the pro-apoptotic protein ANT. Knock-down of misc-1 in C. elegans and OGC in mouse cells induces apoptosis through the caspase cascade. Genetic analysis suggests that MISC-1 controls apoptosis through the physiological pathway mediated by the LIN-35/Rb-like protein. We provide genetic and molecular evidence that absence of MISC-1 increases insulin secretion and enhances germline stem cell proliferation in C. elegans. Our study suggests that the mitochondrial metabolic protein MISC-1/OGC integrates metabolic, apoptotic and insulin secretion functions. We propose a novel mechanism by which mitochondria integrate metabolic and cell survival signals. Our data suggest that MISC-1/OGC functions by sensing the metabolic status of mitochondria and directly activate the apoptotic program when required. Our results suggest that controlling MISC-1/OGC function allows regulation of mitochondrial morphology and cell survival decisions by the metabolic needs of the cell.

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