Misexpression screen delineates novel genes controlling Drosophila lifespan

Donggi Paik; Yeo Gil Jang; Young Eun Lee; Young Nam Lee; Rochelle Yamamoto; Heon Yung Gee; Seungmin Yoo; Eunkyung Bae; Kyung Jin Min; Marc Tatar; Joong Jean Park

doi:10.1016/j.mad.2012.02.001

Misexpression screen delineates novel genes controlling Drosophila lifespan

Donggi Paik, Yeo Gil Jang, Young Eun Lee, Young Nam Lee, Rochelle Yamamoto, Heon Yung Gee, Seungmin Yoo, Eunkyung Bae, Kyung Jin Min, Marc Tatar, Joong Jean Park

Department of Pharmacology

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

In an initial preliminary screen we identified factors associated with controlling . Drosophila aging by examining longevity in adults where EP elements induced over-expression or antisense-RNA at genes adjacent to each insertion. Here, we study 45 EP lines that initially showed at least 10% longer mean lifespan than controls. These 45 lines and a . daughterless (da)-Gal4 stock were isogenized into a . CS10 wild-type background. Sixteen EP lines corresponding to 15 genes significantly extended lifespan when their target genes were driven by . da-Gal4. In each case, the target genes were seen to be over-expressed. Independently derived UAS-gene transgenic stocks were available or made for two candidates: . ImpL2 which is ecdysone-inducible gene L2, and . CG33138, 1,4-alpha-glucan branching enzyme. With both, adult lifespan was increased upon over-expression via the GeneSwitch inducible Gal4 driver system. Several genes in this set of 15 correspond to previously discovered longevity assurance systems such as insulin/IGF-1 signaling, gene silencing, and autophagy; others suggest new potential mechanisms for the control of aging including mRNA synthesis and maturation, intracellular vesicle trafficking, and neuroendocrine regulation.

Original language	English
Pages (from-to)	234-245
Number of pages	12
Journal	Mechanisms of Ageing and Development
Volume	133
Issue number	5
DOIs	https://doi.org/10.1016/j.mad.2012.02.001
Publication status	Published - 2012 May

Bibliographical note

Funding Information:
We thank Dr. Saitoe for CS10 flies, Dr. Monnier for da-GS-Gal4 flies, Szeged Stock Center for some EP lines, and Bloomington Stock Center for Hsp70-Gal4 and S106-GS-Gal4 flies. We also thank Dr. Silverman for his comments on this manuscript. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 20110028200 ). Work from the laboratory of M. Tatar was supported through the National Institute of Aging, NIH (USA) by R01AG024360 and R01AG031152 , and by the Glenn Medical Foundation and the Ellison Medical Foundation .

All Science Journal Classification (ASJC) codes

Ageing
Developmental Biology

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10.1016/j.mad.2012.02.001

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title = "Misexpression screen delineates novel genes controlling Drosophila lifespan",

abstract = "In an initial preliminary screen we identified factors associated with controlling . Drosophila aging by examining longevity in adults where EP elements induced over-expression or antisense-RNA at genes adjacent to each insertion. Here, we study 45 EP lines that initially showed at least 10% longer mean lifespan than controls. These 45 lines and a . daughterless (da)-Gal4 stock were isogenized into a . CS10 wild-type background. Sixteen EP lines corresponding to 15 genes significantly extended lifespan when their target genes were driven by . da-Gal4. In each case, the target genes were seen to be over-expressed. Independently derived UAS-gene transgenic stocks were available or made for two candidates: . ImpL2 which is ecdysone-inducible gene L2, and . CG33138, 1,4-alpha-glucan branching enzyme. With both, adult lifespan was increased upon over-expression via the GeneSwitch inducible Gal4 driver system. Several genes in this set of 15 correspond to previously discovered longevity assurance systems such as insulin/IGF-1 signaling, gene silencing, and autophagy; others suggest new potential mechanisms for the control of aging including mRNA synthesis and maturation, intracellular vesicle trafficking, and neuroendocrine regulation.",

author = "Donggi Paik and Jang, {Yeo Gil} and Lee, {Young Eun} and Lee, {Young Nam} and Rochelle Yamamoto and Gee, {Heon Yung} and Seungmin Yoo and Eunkyung Bae and Min, {Kyung Jin} and Marc Tatar and Park, {Joong Jean}",

note = "Funding Information: We thank Dr. Saitoe for CS10 flies, Dr. Monnier for da-GS-Gal4 flies, Szeged Stock Center for some EP lines, and Bloomington Stock Center for Hsp70-Gal4 and S106-GS-Gal4 flies. We also thank Dr. Silverman for his comments on this manuscript. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 20110028200 ). Work from the laboratory of M. Tatar was supported through the National Institute of Aging, NIH (USA) by R01AG024360 and R01AG031152 , and by the Glenn Medical Foundation and the Ellison Medical Foundation . ",

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AU - Paik, Donggi

AU - Jang, Yeo Gil

AU - Lee, Young Eun

AU - Lee, Young Nam

AU - Yamamoto, Rochelle

AU - Gee, Heon Yung

AU - Yoo, Seungmin

AU - Bae, Eunkyung

AU - Min, Kyung Jin

AU - Tatar, Marc

AU - Park, Joong Jean

N1 - Funding Information: We thank Dr. Saitoe for CS10 flies, Dr. Monnier for da-GS-Gal4 flies, Szeged Stock Center for some EP lines, and Bloomington Stock Center for Hsp70-Gal4 and S106-GS-Gal4 flies. We also thank Dr. Silverman for his comments on this manuscript. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 20110028200 ). Work from the laboratory of M. Tatar was supported through the National Institute of Aging, NIH (USA) by R01AG024360 and R01AG031152 , and by the Glenn Medical Foundation and the Ellison Medical Foundation .

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Misexpression screen delineates novel genes controlling Drosophila lifespan

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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